MTT法评价镧原位改性HMS材料的细胞毒性

Cytotoxicity of La-HMS by MTT

背景:国内外对La-HMS制备工艺的探讨比较多,但缺乏对La-HMS生物安全性评价的研究。目的:制备La-HMS六方介孔材料,并对其进行生物学评价。方法:以长链伯胺为模板剂,在中性条件下合成出具有六方结构的介孔二氧化硅分子筛,以介孔二氧化硅为载体,将镧元素嵌入分子筛骨架,得到La-HMS。将La-HMS粉末与一定量的黏合剂混合并压片,并经高温高压消毒备用。分离培养鼠骨髓间质干细胞,倒置荧光显微镜下观察培养过程中细胞的形态及增殖情况,MTT检测La-HMS细颗粒对小鼠骨髓间质干细胞相对增殖率的影响,评价其细胞毒性。结果与结论:培养1d后,细胞可在La-HMS周围贴壁生长,此后每天细胞生长情况良好,一直保持正常的形态。3d和5d后各培养孔中都未发现脱落的死亡细胞,细胞仍保持原来的形状且数量逐渐增加,且形态良好。MTT检测结果显示,实验制备的La-HMS没有毒性。提示实验用的材料为六方介孔材料,材料的介孔结构利于细胞的黏附,材料符合生物材料细胞毒性要求,具有良好的生物相容性和细胞黏附性,可应用于生物医药方面。

BACKGROUND：There are many studies addressing the preparation of La-HMS,but the research of biological safety evaluation has not been reported.OBJECTIVE：To prepare La-HMS hexagonal mesoporous materials,and to evaluate the biocompatibility of the material.METHODS：Using long-chain primary amine as a template,a hexagonal structure of mesoporous silica molecular sieves was synthetized under neutral conditions,with mesoporous silica as a vector,then lanthanum was embed into zeolite to obtain La-HMS.The La-HMS powder was mixed with a certain amount of adhesives and pressed,then autoclaved.The cells used in this experiment were derived from rat bone marrow mesenchymal stem cells.The morphology and proliferation of the cultured cells were observed under inverted fluorescence microscope,the effects of La-HMS particles on the relative proliferation rate of rat bone marrow mesenchymal stem cells were detected by MTT-assay that could assess the cytotoxicity rapidly.RESULTS AND CONCLUSION：After 1 day of the culture,cells adherently grew around the La-HMS,then the growth was observed in good condition daily,maintaining a normal shape.At 3 and 5 days,there were no exutive died cells found in each culture hole,cells maintained their good shape and the number gradually increased.MTT results showed that the prepared La-HMS was non-toxic.The materials used in the experiment were hexagonal mesoporous materials,the mesoporous structure is conducive to cell adhesion,and the cytotoxicity is in line with the requirement of the biological materials,it possesses good biocompatibility and cellular adhesion,and can be applied to bio-pharmaceutical industry.