纳米银猪脱细胞真皮敷料的细胞毒性评估

Cytotoxiciy of nano-silver/porcine acellular dermis dressings

背景:传统的猪皮敷料具有保护创面的作用,但不具有主动抗感染功能。目的:评估自制纳米银猪脱细胞真皮敷料的体外细胞毒性,评价其作为新型敷料的可行性。方法:用四甲基偶氮唑盐比色法检测自制纳米银猪脱细胞真皮敷料100%和50%的浸提液对小鼠成纤维细胞(L-929细胞)相对增殖率的影响,并评价其细胞毒性。倒置显微镜观察各组细胞形态变化,培养2,4,7d后用酶联免疫检测仪测定溶液吸光度值来评估细胞毒性。结果与结论:L-929细胞在自制纳米银猪脱细胞真皮敷料的浸提液中增殖良好,其细胞毒性实验显示细胞毒级为1级,实验组与阴性对照组之间的吸光度值差异均无显著性意义(P>0.05)。说明自制纳米银猪脱细胞敷料无细胞毒性,作为新敷料具有可行性。

BACKGROUND：Traditional porcine dermal dressing can protect wounds,but it has not the properties of bacteriostasis efficacy.OBJECTIVE：To assess in vitro toxicity of self-made nano-silver porcine acellular dermal dressing and to evaluate the feasibility of a new type of dressing.METHODS：L-929 mouse fibroblasts were used to detect the cell relative proliferation rate in the extract from the nano-silver porcine dermal dressing 100% and 50% by MTT-assay,the toxicity of porcine dressing was also determined.Morphology of the cells was observed under inverted microscope.The absorbance value was detected using enzyme-linked immunospot assay at 2,4,7 days of the culture,and the cytotoxicity was assessed.RESULTS AND CONCLUSION：The L-929 cells proliferated well in the extract of nano-silver porcine acellular dermis dressing,and the cell toxicity was graded as 1.There was no significant difference in the absorbance value between the experimental group and the negative control group（P 0.05）.The nano-silver pig acellular dermis dressing shows no evident cytotoxicity and is feasible to serve as a new type of wound dressing.