六氟硅酸铵溶液对牙本质小管的封闭作用

Occlusion of dentinal tubules by(NH_4)_2SiF_6 solution

背景:各种封闭牙本质小管和降低牙本质小管内液体流动性的方法具有不同程度的治疗效果。目的:观察六氟硅酸铵溶液对牙本质小管的封闭作用,探讨六氟硅酸铵溶液治疗牙本质敏感症的可能机制。方法:选择离体第3磨牙制作离体牙本质盘12块,并分预处理4组。分别经0.29mol/LEDTA内浸置2min;6%柠檬酸内浸置2min;50%柠檬酸内浸置2min;蒸馏水超声清洗20min预处理。以上每组随机再分为3个亚组,分别应用试验药物0.476mol/L六氟硅酸铵溶液、对照药物2.36mol/L银氟二铵溶液或生理盐水处理3min,然后应用扫描电镜技术观察牙本质小管经试验药物处理前后开放情况,经光电子能谱分析氟、硅、银、磷、钙组成比。结果与结论:扫描电镜下可以看到经六氟硅酸铵溶液和银氟二铵溶液处理后牙本质小管开放面积明显减小;经六氟硅酸铵溶液处理后牙本质小管中所形成的沉淀物中含有硅、钙和磷,呈现为一种硅磷酸钙盐复合体,经银氟二铵溶液处理后牙本质小管仍处于开放状态,表面有不同程度的沉积物覆盖;两种含氟药物处理后的牙本质小管开放面积存在显著性差异。结果提示,六氟硅酸铵溶液有明显封闭牙本质小管作用,且封闭效果随酸性预处理溶液的pH值降低而降低。

BACKGROUND：Various methods for closing dentinal tubules and reducing fluid flow in dentinal tubule have different treatment effects.OBJECTIVE：To observe the occlusion effect of（NH4）2SiF6 solution on dentinal tubules,and to determine the possible mechanisms underlying（NH4）2SiF6 solution in dentin hypersensitivity treatment.METHODS：Twelve free dentinal discs were made from free third molars,and divided into four pretreatment groups.The teeth were soaked in pretreatment solution for 2 minutes,namely 0.29 mol/L EDTA,6% citric acid,50% citric acid,and rinsed ultrasonically with deionized water 20 minutes,respectively.Every above-mentioned group was randomly assigned into experimental group,control group and blank group,respectively.The teeth of three groups were treated for 3 minutes by experimental solution,namely 0.476 mol/L（NH4）2SiF6,2.36 mol/L Ag（NH3）2F solution and physiological saline,respectively.The opening on the dentinal tubules before and after treatment was observed using scanning electron microscope.The constituent ratio of fluorine,silicon,silver,phosphor and calcium distributing the premolars surface was analyzed by the photoelectron spectrometer.RESULT AND CONCLUSION：Under scanning electron microscope,the opening area of dentinal tubules significantly decreased after the（NH4）2SiF6 and Ag（NH3）2F solution treatment;the sediments formed dentinal tubules contained silicon,calcium and phosphorus,presented as a complex of calcium phosphate silicon following（NH4）2SiF6 treatment,dentinal tubules remained open state after Ag（NH3）2F solution treatment and showed various levels of sediments on surface;there were significant differences in dentinal tubules open area between two kinds of fluorine-containing drugs treatments.The results suggest that the occlusion effect on dentin tubules is significant by using（NH4）2SiF6 solution,and reduced with reducing pH value of pretreatment solution.