薄层色谱法与苯酚硫酸法联用控制黄芪多糖粗粉质量

Controlling Quality of Astragalus Polysaccharide Meal by Combined TLC and Phenol-Sulfuric Acid Method

[目的]建立一种有效控制黄芪多糖粗粉质量的方法。[方法]用薄层色谱法半定量测定了自制黄芪多糖粗粉中葡萄糖和蔗糖的含量,用苯酚硫酸法测定了其中总糖的含量,并初步研究了影响苯酚硫酸法测量重复性的因素。[结果]该批黄芪多糖粗粉中,葡萄糖、蔗糖和总糖的含量分别为0.83%、6.67%、42.00%。[结论]该研究所用的方法简便、快速,可以作为现场检测黄芪多糖制剂的方法。

[Objective]To establish an effective method for controlling the quality of Astragalus polysaccharide meal. [Method]The assay of glucose and sucrose in the self extracted Astragalus polysaccharide meal was semi-quantitatively determined by TLC and the assay of total sugar was determined by phenol-sulfuric acid method. The influencing factors of measuring reapeatability was preliminarily studied. [Results]The assay of glucose,sucrose and total sugar in the Astragalus polysaccharide meal were 0. 83% ,6. 67% ,42. 00% respectively. [Conclusion]The methods in this research are simple and rapid,and can be used as a site detection technique for Astragalus polysaccharide preparations.