摘要
[目的]建立麻花艽(Gentiana straminea Maxim.)不同部位中异荭草素的RP-HPLC检测方法。[方法]色谱条件为:HypersilODS柱(4.6mm×250.0mm,5μm),以40%甲醇-水溶液(含0.02%磷酸)等度洗脱,流速1ml/min,柱温35℃,检测波长254nm。[结果]在设定的色谱条件下,异荭草素与其他组分达到基线分离,线性范围为0.0899～0.539.0μg,r=0.9999。加标回收率为99.7%,RSD为0.49%。[结论]方法快速、准确,结果可靠。
[Objective]The research aimed to establish the detection method of isoorientin from different parts of Gentiana straminea Maxim. by reversed phase -HPLC. [Method]The chromatographic conditions were as follows：Hypersil ODS column （4. 6 mm × 250. 0 mm,5 μm）,40% methanol-H2O（containing 0. 02 % phosphoric acid）as a mobile phase,with flow rate of 1 ml/min,column temperature at 35 ℃,detection wavelength at 254 nm. [Result]Under the chromatographic conditions,isoorientin and other components reached the baseline separation. The linear range was 0. 089 9 -0. 539 0 μg,r = 0. 999 9. The addition recovery was 99. 7% ,with RSD of 0. 49%. [Conclusion]The method is rapid and accurate and the results are reliable.
出处
《安徽农业科学》
CAS
北大核心
2010年第14期7341-7342,共2页
Journal of Anhui Agricultural Sciences
基金
青海省科技厅项目(2006-N-141)
