大豆质核互作雄性不育系NJCMS3A双亲雄性育性基因的SSR标记

Mapping Male Fertility Gene with SSR Markers in Parents of Cytoplasmic-Nuclear Male-Sterile Line NJCMS3A in Soybean

利用大豆质核互作雄性不育系NJMCS3A的质、核供体亲本N21566和N21249构建F_2和BC_1F_1育性分离群体进行雄性育性的遗传分析与基因定位。结果表明,F1正反交可育,F2和BC1F1的可育株与不育株分离比例经χ2测验分别符合3∶1和1∶1,表明NJCMS3A供体亲本雄性育性由一对基因控制,可育等位基因为显性。该基因可能是NJCMS3A的一个恢复基因。选用793对SSR引物对F_2和BC_1F_1群体分别进行育性基因定位,发现该育性基因位于O连锁群上,在Satt331和Satt477标记之间,与Satt331、CSSR133和Satt477标记距离的次序一致,分别为8.1～10.4、11.4～16.4和13.3～19.2cM。

Utilization of the three lines [cytoplasmic-nuclear male-sterile （CMS） line, maintainer line, and restorer line] in pro- ducing hybrids with heterosis has been very successful in a number of crops including soybean. The soybean CMS line NJCMS3A was developed through six consecutive backcross generations with N21566 as cytoplasm source and N21249 as nuclear donor. The objective of our study was to reveal the genetic mechanism and mapping the male fertility gene in the two parents of the CMS line NJCMS3A, i.e. N21566 and N21249. The male fertility of （N21566×N21249） F2 and BC1FI showed that the segregation ratio of fertile to sterile plants fitted the expected ratios of 3：1 and 1：1, respectively, indicating one pair of gene conferring male fertility in the parents of NJCMS3A with dominance allele in N21249 being male fertile. This gene might be one of the restorer genes for the male sterility of NJCMS3A. The mapping results on BCIF1 and F2 showed that out of the 793 randomly selected SSR markers, Satt331, CSSR133 and Satt477 on linkage group O were linked to the male fertility gene Rf with their genetic distances of 8.1-10.4, 11.4-16.4, and 13.3-19.2 cM, respectively, in a same order and its location between Satt331 and Satt477.