OSAHS患者外周血Th1/Th2细胞比例变化及临床意义

Changes of peripheral blood helper T cell in patients with obstructive sleep apnea hypopnea syndrome

目的探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者外周血Th1/Th2细胞比例变化及临床意义。方法经多导睡眠图确诊OSAHS患者60例,按照呼吸暂停低通气指数(AHI)分为轻度OSAHS组(n=18),中度OSAHS组(n=22),重度OSAHS组(n=20)。对照组为年龄、性别、BMI匹配,经多导睡眠监测除外OSAHS者20例。用流式细胞术检测各组Th1/Th2细胞比例并进行各组间比较。对OSAHS患者睡眠期AHI和Th1细胞比例进行相关性分析。结果 OSAHS组Th1比例明显高于对照组[(18.2±5.4)%和(12.5±3.8)%,P<0.05],其中重度OSAHS组增高较对照组尤为明显[(21.8±4.6)%和(12.5±3.8)%,P<0.01]。OSAHS患者Th1细胞比例与AHI呈正相关(r=0.668,P<0.01)。Th2比例差异在各组间均无统计学意义(P>0.05)。结论 OSAHS患者Th1细胞激活程度显著增高,且与OSAHS的严重程度相关。

Objective To investigate the changes of Peripheral Blood Helper T＇ cell in patients with obstructive sleep apnea hy- popnea syndrome （OSAHS）. Methods Based on apnea hypopnea index （AHI） measured by polysomnography, 60 patients with OSAHS were divided into mild OSAHS group （L,18 cases）,moderate OSAHS group （M,22 cases） and severe OSAHS group （ H, 20 cases）. 20 healthy subjects with matched age, gender and body mass index were selected as control. The ratio of Thl and Th2 in peripheral blood was detected by flow cyt ometry. Results The ratio of Thl in patients with OSAHS was higher than it in con- trol group[（18.2±5.4） %vs （12.5＋3.8） % ,P〈0.05）] ,The ratio of Thl in group H increased sharply[group H vs control （21. 8±4.6）%vs （12.5±3.8）% ,P〈0. 01）]. The ratio of Thl positively correlated to AHI（r=0.668,P〈0.01）.There were no difference of the ratio of Th2 in each group. Conclusion The ratio of Thl is significantly increased in OSAH＇S patients, which is as- sociated with the severity of OSAHS.