摘要
目的研究黄芩甙对大鼠肝星状细胞(HSC)凋亡的影响,探讨黄芩甙抗肝纤维化的机制。方法采用胶原酶循环灌流法分离肝星状细胞。以不同浓度黄芩甙作用后,通过相差显微镜观察细胞形态变化,用MTT法、溴乙锭/吖啶橙荧光染色法、流式细胞术检测黄芩甙对活化的HSC凋亡的影响。结果黄芩甙显著促进活化的HSC凋亡,此作用随黄芩甙浓度增加而增强,呈药物浓度依赖关系。结论黄芩甙可以促进HSC凋亡,减少活化HSC的总量,发挥其抗肝纤维化作用。
Objective To study the effects of Baiealin on the apoptosis of cultured rat hepatic stellate cells （HSCs）. Methods HSCs were cultured with different concentrations of Baicalin 0,75,150,300,600,1 200 μg/mL for 24 hours. Cell apoptosis was de- tected with phase contrast light microscopy,MTT colorimetric assay, AO/EB fluorescent staining and flow cytometry. Results Ba- icalin markedly increases apoptosis of HSCs,and the effect depends on the drug dose. Conclusion Baicalin can cause HSCs apopto- sis directly, which might be one of the mechanisms of Baidalin in reducing liver fibrosis.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第13期1665-1666,F0003,共3页
Chongqing medicine
关键词
肝纤维化
黄芩甙
肝星状细胞
凋亡
liver fibrosis
baicalin
hepatic stellate cell
apoptosis
