云南松幼苗上红菇类菌根真菌的物种多样性及其菌根形态

Species Diversity of Russuloid Mycorrhizae-forming Fungi on Pinus yunnanensis Seedlings and the Mycorrhizal Morphology

以滇中1～2年生云南松幼苗为研究对象,观察鉴定与其共生的红菇属真菌外生菌根。形态观察发现了6种形态型(morphotypes)。本文对这6种形态型的外观和显微特征进行了详细描述,尤其强调了菌套形态特征。对rDNAITS片段比对分析表明,6种形态型对应6种红菇属真菌,它们分别是与Russula li-vescens、R.violeipes、R.densifolia、R.nigricans、R.sanguinea及R.nauseosa相近的红菇种类。本研究表明,形成的菌根及其菌套和囊状体的特征在红菇属真菌的系统分支间表现出较为稳定的差异。这一研究与前人对该属真菌的菌根形态及分类学研究基本吻合。红菇属真菌是云南松1～2年幼苗期的主要共生真菌类群。

Russuloid ectomycorrhizae on 1-2 years-old seedlings of Pinus yunnanensis collected from the central Yunnan,China were investigated. Six morphotypes were recognized by macro-and anatomical-morphological approaches as well as molecular analyses. The six morphotypes were confirmed to represent six phylotypes by matching rDNA ITS sequences. The fungal partners in the six morpho-phylotypes are those closely related with Russula livescens,R.violeipes ,R.densifolia ,R.nigricans ,R.sanguinea and R.nauseosa respectively. The correlation between the morphology of mantles and cystidia and the phylogenetic clades is further supported in our work. The framework,in which this report is included,shows that russuloid mycorrhizae are one of the most dominant representative ectomycorrhizae formed on 1-2 years seedlings of Pinus yunnanensis.