异基因造血干细胞移植前肿瘤和移植后第二肿瘤中survivin,MMP2,TIMP1,CD44和nm23的表达变化

Expressions of surviving,MMP2,TIMP1,CD44 and nm23 of two tumors originating from different immunosurveillance in the same patient

目的探讨在不同免疫状态下发生的移植前肿瘤和移植后第二肿瘤,其肿瘤粘附、扩散及存活相关因子Survivin,MMP2,TIMP1,CD44和nm23的表达变化。方法急性淋巴细胞白血病骨髓标本和异基因造血干细胞移植后发生的淋巴瘤标本均来自于同一个病人,应用免疫组织化学方法对两份肿瘤标本进行了Survivin,MMP2,TIMP1,CD44和nm23的表达差异对比研究。结果移植前患者的白血病骨髓标本细中,Survivin,MMP2,TIMP1,CD44和nm23明显强表达,移植后发生的淋巴瘤标本细胞中的上述分子不表达或极弱表达。结论接受异基因外周造血干细胞移植的患者发生的第二肿瘤,其与恶性肿瘤存活、黏附和转移有关的分子Survivin、MMP2、TIMP1、CD44和nm23的表达均较普通免疫状态下出现表达抑制现象。

Objective To explore the differences in the survival,adhesion and diffusion capacity between different carcinomas originating from different immunosurveillance in the same patient.Methods The expressions of survivin,MMP2,TIMP1,CD44,and nm23 proteins were detected immunohistochemically in a patient with acute lymphoblastic leukemia and lymphoma after allogeneic blood stem cell transplantation.Results Survivin,MMP2,TIMP1,CD44,and nm23 proteins were positive in acute lymphoblastic leukemia samples obtained before transplantation and negative in the lymphoma tissue occurring after the transplantation.Conclusion The expressions of survivin,MMP2,TIMP1,CD44,and nm23 proteins vary between the two carcinomas originating from different immunosurveillance in the same patient.