光动力联合紫杉醇对食管癌细胞株Eca-109增殖的影响

Inhibitory effect of photodynamic therapy combined with paclitaxel on the proliferation of esophageal carcinoma Eca-109 cells

目的探讨血卟啉衍生物联合紫杉醇注射液对人食管癌细胞Eca-109体外光动力疗法效应的影响。方法将食管癌细胞株Eca-109分为8组:对照组、化疗组、化疗+高剂量光敏剂治疗组、化疗+中剂量光敏剂治疗组、化疗+低剂量光敏剂治疗组、高剂量光敏剂治疗组、中剂量光敏剂治疗组和低剂量光敏剂治疗组。种板孵育24 h,检测肿瘤细胞的存活率;于化疗方案组加入紫杉醇作用12 h,再往含光敏剂实验组分别加入血卟啉衍生物高、中、低剂量,4 h后行光动力照射,照光前后荧光显微镜下观察细胞凋亡形态,照光后继续培育24 h,开始行MTT法检测食管癌细胞增殖的抑制率;流式细胞仪检测细胞凋亡率。结果血卟啉衍生物浓度越高食管癌细胞显示的荧光强度越强,荧光强弱与细胞的活性成正比,与紫杉醇联合组则可见到更多凋亡细胞。在肿瘤细胞的生长抑制率、细胞凋亡率方面,化疗+高剂量光敏剂光动力治疗组与空白对照组、单纯化疗组及低剂量光敏剂光动力治疗组相比,差异均有显著意义(P<0.01);含光敏剂大剂量组与小剂量组相比,差异有显著意义(P<0.05);化疗组及光动力组与空白对照组相比,差异有显著意义(P<0.05)。结论光动力联合紫杉醇化疗对人食管癌细胞细胞株Eca-109增殖有协同抑制作用,二者可协同诱导人食管癌细胞株发生凋亡。

Objective To investigate the inhibitory effect of photodynamic therapy（PDT） in combination with paclitaxel（PCT） on proliferation in esophageal carcinoma Eca-109 cells line.Methods Eca-109 cells were treated with PCT alone,HPD alone at different doses,or their combinations.For the combined treatments,the cells were exposed to PCT for 12 h followed by incubation with HPD at high,middle or low concentrations for 4 h.PDT was then performed on these treated cells and fluorescence microscopic observation was made before and after PDT.The cell survival was measured by MTT assay,and the cell apoptosis rate analyzed by flow cytometry after a 24-h cell incubation following PDT.Results The fluorescence excitation of the cells was weakened after PDT.Combined treatments resulted in significantly lowered cell survival rate and increased cell apoptosis rates as compared to those of the control cells and the cells treated with PCT alone and low-dose HPD（P0.01）.Significant differences were also noted among the cells exposed to HPD at different concentrations（P0.05）.Conclusion PDT combined with PCT have significant synergetic effects in inhibiting the proliferation of human esophageal carcinoma cells and inducing their apoptosis in vitro.