TGF-β1和E-cadherin在卵巢癌原发灶和转移灶组织中的表达与意义

Expression of TGF-β1 and E-cadherin in primary and metastatic ovarian carcinoma

目的探讨卵巢癌原发灶和转移灶中转化生长因子-β1(TGF-β1)和上皮型钙粘素(E-cadherin)表达的变化及其对卵巢癌转移意义。方法采用免疫组化方法检测58例卵巢癌原发灶和配对的38例转移灶,49例良性卵巢癌上皮性肿瘤和37例正常卵巢上皮组织的TGF-β1和E-cadherin蛋白的表达情况,分析两者表达的相关性及与卵巢癌临床病理指标的关系,观察二者表达在原发灶和转移灶的差异。结果 TGF-β1蛋白在卵巢癌组表达阳性率为67.2%,显著高于良性肿瘤组(28.6%)和正常组(18.9%)(P<0.05)(χ2=26.94,P<0.001);而E-cadherin在卵巢癌组的表达阳性率为43.1%,显著低于良性肿瘤组(93.9%)和正常卵巢组(94.6%)(χ2=46.54,P<0.001)。TGF-β1在原发灶的表达与卵巢癌的FIGO分期、淋巴结转移和腹水有关(P均<0.05),E-cadherin在卵巢癌原发灶的表达分别和卵巢癌的分化和淋巴结转移有关(P均<0.05),TGF-β1和E-cadherin在卵巢癌原发灶的表达均低于转移灶(χ2=4.70,P=0.03;χ2=5.91,P=0.015),且二者在原发灶的表达呈负相关(Kappa值=-0.32,P=0.01)。结论 TGF-β1和E-cadherin与卵巢癌转移的关系较为密切,有可能成为卵巢癌控制转移的靶点。

Objective To detect the expression of the protein of TGF-β1 and E-cadherin in the primary and metastatic lesions of ovarian carcinoma and explore the mechanism of the metastasis of ovarian carcinoma.Method Immunohistochemistry （IHC） was performed to detect the expression of TGF-β1 and E-cadherin proteins in primary and metastatic ovarian carcinoma,benign epithelial ovarian tumor and normal ovarian tissue.Results The expression of TGF-β1 was significantly higher in ovarian carcinoma（67.2%） than in benign tumors（28.6%） and normal ovarian tissue（18.9%）（χ2=26.94,P0.001）,but E-cadherin expression showed a reverse pattern.TGF-β1 expression in the primary ovarian carcinoma carcinoma was associated with the FIGO stage,lymph metastasis and ascites of the tumor（P=0.01,P=0.01,and P=0.04,respectively）.E-cadherin expression in the tumor was associated with the differentiation（P=0.02） and lymph metastasis of ovarian carcinoma（P=0.04）.The expressions of TGF-β1 and E-cadherin were all significantly lower in the primary tumors than in the metastatic tumor（χ2=4.70,P=0.03;χ2=5.91,P=0.015）.A significant correlation was found between the expressions of the TGF-β1 and E-cadherin in the primary carcinoma（Kappa value of-0.32,P=0.01）.Conclusion TGF-β1 and E-cadherin are closely associated with the metastasis of ovarian carcinoma and might be potential targets for controlling the metastasis of ovarian carcinoma.