神经梅毒患者脑脊液CD4＋ CD25high调节性T细胞的研究

CD4＋ CD25high regulatory T cell frequency in cerebrospinal fluid of patients with neurosyphilis

目的 研究神经梅毒患者脑脊液中的淋巴细胞的表型,探讨其在神经系统免疫损伤机制中的作用.方法 采用流式细胞术检测12例神经梅毒患者和20例潜伏梅毒患者脑脊液CD4＋T细胞和CD4＋ CD25hhigh调节性T细胞（Tr）的比例与变化.结果 神经梅毒患者脑脊液白细胞数量明显升高,其中淋巴细胞比例较高;淋巴细胞以CD4＋ T细胞为主,而其中CD4＋CD25highTr细胞的比例显著低于潜伏梅毒患者.结论 神经梅毒脑脊液中增加的白细胞主要为CD4＋ T细胞,表明CD4＋T细胞可能参与神经梅毒的发病,而CD4＋ CD25high Tr细胞表达减少表明其在维持神经系统局部免疫自稳中可能发挥重要作用.

Objective To analyze the phenotypes of lymphocytes in cerebrospinal fluid derived from the patients with neurosyphilis. Methods Samples of cerebrospinal fluid from 12 patients with neurosyphilis and 20 patients with latent syphilis were collected and analyzed by flow cytometry for CD4 and CD25 expression. Results There was a significant increase in the number of white blood cells in the cerebrospinal fluid of patients with neurosyphilis. FACS analysis showed that most leukocytes were lymphocytes predominated with CD4 ＋ T cells in neurosyphilis patients which were almost 4 times more than that in latent syphilis. However, there was a significant decrease in the proportion of CD4＋ CD25high regulatory T cells （Tr） in neurosyphilis patients compared with that in latent syphilis patients. Conclusion A dramatic increase in CD4＋ T cell frequency suggested its pathogenic role in neurosyphilis, whereas a decrease in CD25high Tr frequency indicated that CD4 ＋ CD25high Tr cells might play an important role in immune homeostasis of central nervous system.