桂枝挥发油不同萃取方法的比较研究

Comparative Study on Different Methods of Extracting Essential Oil from Ramulus Cinnamomi

[目的]为工业提取桂枝挥发油筛选方法。[方法]以桂皮醛为标准品,利用薄层层析法、高效液相色谱法进行含量分析,对超临界CO2萃取法和水蒸气蒸馏法提取的桂枝挥发油进行得率和纯度比较。[结果]在0.5249～2.0994μg/ml浓度范围内,桂皮醛含量与峰面积呈线性关系,其回归方程为:Y=6.0333×106X+0.4573×106,r=0.9998;超临界CO2萃取法提取的桂枝挥发油得率较水蒸气蒸馏法提高了7.33倍,且纯度高。[结论]采用超临界CO2萃取法提取桂枝挥发油可以提高提取率。

[Objective] A new method for the industrial extraction of essential oil from Ramulus Cinnamomi was selected.[Method] With cinnamaldehyde as standard substance,the content analysis was conducted by TLC and HPLC.The yield and purity of essential oil extracted from Ramulus Cinnamomi by supercritical-CO2 extraction（SFE-CO2）method were compared with that by steam distillation.[Result]In the concentration range of 0.524 9-2.099 4 μg/ml,cinnamaldehyde content showed a linear relationship with peak area.And their linear regression equation was Y=6.033 3×10^6 X＋0.457 3×10^6,r=0.999 8.The yield of essential oil from Ramulus Cinnamomi by supercritical-CO2 extraction（SFE-CO2）method increased 7.33 times than that by steam distillation.And the purity of essential oil from Ramulus Cinnamomi by supercritical-CO2 extraction（SFE-CO2）method was higher.[Conclusion] Extracting essential oil from Ramulus cinnamomi by SFE-CO2 method could enhance the extracting yield.