罹病棕尾别麻蝇体上蜡蚧轮枝菌的生物学特性与致病性研究

Biological Characteristics and Pathogenicity of Verticillium lecanii Isolated from Naturally Died Boettcherisca peregrine

[目的]为找到适合蝇类生物防治的病原微生物,对罹病棕尾别麻蝇Boettcherisca peregrine体上的病原物进行分离纯化。[方法]利用光学显微镜镜检菌丝和分生孢子的形态,根据病原菌的培养性状、菌丝和分生孢子的形态进行鉴定,并对其生物学特性和致病性进行初步研究。[结果]棕尾别麻蝇体上的病原物为蜡蚧轮枝菌Verticillium lecanii,将该菌株编号为KMZW-1。该菌株菌落生长最适培养基为PDA培养基,最适温度为29℃,最适pH值为6.0。其孢子悬浮液对棕尾别麻蝇、丝光绿蝇、家蝇、酪蝇、黑腹果蝇成虫的致死中浓度(LC50)分别为9.50×105、4.58×107、4.06×107、4.10×103和1.05×107分生孢子/ml,致死中时间(LT50)分别为6.86、8.17、8.16、8.12和3.22d。[结论]蜡蚧轮枝菌KMZW-1是一种对5种蝇类成虫具有活性的病原菌。

[Objective]In order to find the pathogenic microorganisms suitable for biological control of filth flies,the pathogenic microorganism was isolated from the dead fly,Boettcherisca peregrine.[Method]The conidia and mycelia were observed by optical microscope.The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia,and its biological characteristics and pathogenicity were preliminarily studied.[Result]The results showed that the pathogenic microorganism isolated from the dead fly was one new strain of Verticillium lecanii.The new strain of V.lecanii was numbered as KMZW-1.The colonies of V.lecanii KMZW-1 grew fastest on potato dextrose agar medium（PDA）at 29 ℃ and pH value 6.The LC50 of its spore suspension to the adults of B.peregrine,Lucilia sericata,Musca domestic,Piophila casei and Drosophila melanogaster were 9.50×10^5,4.58×10^7,4.06×10^7,4.10×10^3 and 1.05×10^7 conidia/ml,respectively.And the LT50 were 6.86,8.17,8.16,8.12 and 3.22 d,respectively.The virulence of V.lecanii KMZW-1 to five fly species is good and it can be used to biological control of filth flies.[Conclusion]V.lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species.