马鹿粪便样品微卫星基因型的可靠性评价

Assessing Reliability of Microsatellite Genotypes from Wapiti Fecal Samples

[目的]评价马鹿粪便DNA的微卫星基因型可靠性,为后续保护遗传学方面的研究提供参考。[方法]选择7个多态性较高的微卫星位点,扩增167份马鹿粪便DNA,通过3～4次的重复扩增统计等位基因丢失率和假等位基因出现率。[结果]7个位点的等位基因丢失率平均为0.034,假等位基因出现率平均为0.023。[结论]对于杂合体,3～4次的重复扩增可以满足基因型后续分析要求,而纯合体需要7次以上的重复扩增。

[Objective]The aim was to assess reliability of microsatellite genotypes from wapiti fecal DNA in order to offer researching reference for conservation genetics in the future.[Method]Using 7 high variable loci,167 fecal DNA were genotyped,the ratio of allele dropout and false allele by 3-4 times repeat amplification was estimeated.[Result]The average ratio for allele dropout was 0.034 in 7 loci,the false allele was 0.023.[Conclusion]The method of 3-4 times repeat amplification may be used to assess the genotypes of the heterozygote,while 7 times or more would be carried in the homozygote.