摘要
目的探讨二肽基肽酶Ⅲ(dipeptidyl peptidaseⅢ,DPPⅢ)在正常大鼠眼球各组织中的免疫表达。方法选取正常的两性Wistar大鼠,应用抗生物素蛋白-生物素-过氧化物酶复合体法检测DPPⅢ在正常大鼠眼球各组织中的免疫表达。结果 DPPⅢ在角膜上皮细胞、角膜内皮细胞、睫状体无色素上皮细胞、虹膜色素上皮细胞、晶状体上皮细胞、晶状体纤维细胞、视网膜神经纤维层、视网膜神经节细胞、视网膜外丛状层、视网膜色素上皮细胞中免疫组织化学染色均呈阳性。结论 DPPⅢ主要存在于Wistar大鼠眼球的角膜上皮细胞、角膜内皮细胞、睫状体无色素上皮细胞、虹膜色素上皮细胞、晶状体上皮细胞、晶状体纤维细胞、视网膜神经纤维层、神经节细胞、视网膜外丛状层、视网膜色素上皮细胞。
Objective To investigate the immune expression of dipeptidyl peptidase Ⅲ（DPP Ⅲ） in the each tissues of normal rat eyeball.Methods The normal Wistar rats of both sexes were chosen,the immune expression of DPP Ⅲ in the each tissues of normal rat eyeball were investigated with ABC immunohistochemical method.Results DPP Ⅲ were all found positively by immunohistochemical method in corneal epithelial cells,corneal endothelial cells,ciliary body non-pigment epithelial cells,iris pigment epithelial cells,lens epithelial cells,lens fibroblasts,retinal nerve fiber layer,retinal ganglial cells,retinal outer plexiform layer,retinal pigment epithelial cells.Conclusion DPP Ⅲ are primarily in the corneal epithelial cells,corneal endothelial cells,ciliary body non-pigment epithelial cells,iris pigment epithelial cells,lens epithelial cells,lens fibroblasts,retinal nerve fiber layer,retinal ganglion cells,retinal outer plexiform layer,retinal pigment epithelial cells of Wistar rats.
出处
《眼科新进展》
CAS
北大核心
2010年第7期616-617,621,共3页
Recent Advances in Ophthalmology
基金
教育部留学回国人员科研启动基金资助课题(2005)
长春市科技发展计划资助课题(编号:03-1188527)
吉林省科技发展计划项目(编号:200705233)~~
