膜纯化-分光光度法测定侧柏叶总黄酮含量

An Applied Study on Measurement of General Flavone of Cacumen Biotae by Spectrophotometric Determination Combined with Membrane based

将膜分离技术与植物有效成分分析方法结合，提出以二醋酸纤维素膜（ＣＡＭＦＭ）膜纯化－分光光度法取代蒸干转溶法测定侧柏叶提取液中侧柏叶总黄酮含量。与蒸干转溶法相比，ＣＡＭＦＭ膜纯化－分光光度法具有除杂效率高、分析过程无相变、有效成分理化性质稳定、分析操作简便、分析结果重复性好、准确度较高的特点。

Combined with technology of membrane separation and analytical method of plant effective components, a new analytical method of spectrophotometric determination combined with membrane based purification is put forward to determine the content of general flavone in extracted liquid of Cacumen Biotae instead of traditional evaporating redissolving method. Compared with evaporating re dissolving method, the advantages of membrane based spectrophotometric determination consist in high efficiency of purification, no phase inverse during analysis, simplified analytical operation, satisfied repetition of analytical results and high precision.