黄腹山鹪莺和纯色山鹪莺性别的分子鉴定

Molecular Sexing of the Yellow-bellied Prinia and Plain Prinia

黄腹山鹪莺Prinia flaviventris和纯色山鹪莺P.inornata共同体现出一个特点,其繁殖期尾羽短于其冬季尾羽,但由于两者的雄雌外形相似而难以野外鉴别。为此,我们采用CHD基因法对二者的性别进行鉴定,并通过解剖对鉴定结果进行验证。结果发现:1)P2/P8引物适用于这两个物种的性别鉴定,而2550F/2718R不适用;2)分子方法鉴定结果与解剖鉴定结果完全相符;3)使用非伤害性取样法拔取的羽毛中提取的DNA具有同样效果。为此,我们认为使用P2/P8引物对这两个物种进行性别鉴定可靠,具快速鉴定的效果,而2550F/2718R引物可能不适用于莺科鸟类的性别鉴定。

The yellow-bellied prinia （Prinia flaviventris） and plain prinia （P. inornata） both possess a unique charateristic in that the tail of the breeding plummage is shorter than the winter plummage, which may contradict sexual selection theory. However, sex identification of these species is so difficult that further research is getting harder in the field. So we applied the CHD gene to sex the species. We found that： 1 ） Primer 2550F/2718R was not applicable for sexing the two species but primer P2/P8 was ; 2） Results of molecular sexing were consistent with anatomizing results ; 3） DNA could be also extracted from yellow-bellied prinia and plain prinia and primer 2550F/2718R might not be applicable for the sexing of the birds in Cisticolidae.