利用特异引物和直接测序法鉴定圆鼻巨蜥和孟加拉巨蜥

Application of Species-specific PCR Primers and DNA Sequencing in the Forensic Identification of Two Monitors

利用特异引物扩增法和直接测序法对圆鼻巨蜥Varanus salvator和孟加拉巨蜥Varanus bengalensis进行了物种鉴定。设计通用引物和特异引物扩增COI基因部分序列,根据电泳结果和产物的blast比对结果,成功鉴定两种巨蜥,并对这两种方法的使用范围进行了探讨,解决了实际执法中两种巨蜥物种鉴定的需要。

In the paper, two species of monitors, Varanus salvator and Varanus bengalensis, were identified using two methodologies of species-specific PCR primers and DNA sequencing. Partial fragments of the COI gene were amplified from two monitor lizards using universal primers and species-specific primers. The results showed that species-specific primers and universal primers could be applied in distinguishing the two monitors. In addition, the applicable condition of the two identification methodologies were discussed. These methodologies satisfy the needs of species identification for police cracking down on smuggling.