纳米羟基磷灰石的精氨酸表面修饰及其与基因的结合性能

Arginine modification and gene binding property of hydroxyapatite nanoparticles

采用水热合成法制备经精氨酸表面修饰的羟基磷灰石纳米颗粒(Arg-nHAP)。利用透射电镜(TEM)、Zeta电位分析仪、傅立叶红外光谱仪和X射线衍射(XRD)对Arg-nHAP的形貌、结构、晶粒粒径和Zeta电位进行表征,并将其与质粒DNA(pEGFP-N1)进行凝胶电泳实验,研究Arg-nHAP对基因的结合和保护性能。结果表明:制备的Arg-nHAP粒径较均匀,约为50nm～80nm,分散性良好;在pH=7.4时,纳米颗粒的表面净电荷均值为30.89mV,可与带负电荷的质粒DNA通过静电结合快速形成纳米颗粒-质粒DNA复合物,对结合的DNA起到明显的保护作用;经精氨酸表面修饰的Arg-nHAP可成为一种有效的基因结合载体。

An arginine modifying nano-hydroxyapatite（nHAP） was synthesized by hydrothermal synthesis.The morphology,structure,crystallite size and Zeta potential of the Arg-nHAP were characterized by X-ray diffractometry（XRD） ,transmission electron microscopy（TEM） ,Fourier transform infrared（FTIR） and Zeta potential analyzer.The binding and protecting properties of Arg-nHAP to DNA were tested by electrophoresis experiment.The results show that the Arg-nHAP is very fine and well distributes,the mean diameter of the nanoparticles is 50-80 nm and its Zeta potential is about 30.89 mV at pH of 7.4.Arg-nHAP/DNA nanocomplexes can be formed rapidly by electrostatic interaction of nanoparticles with DNA having negative electrons.The nHAP modified with arginine can effectively bind and protect DNA and may be used as a potential gene carrier.