小鼠腹腔注射阿霉素诱导骨髓细胞微核率的最佳时间—剂量效应分析

A Study of the Optimal Time-Dose Effect of Doxorubicin on Inducing the Micronucleus Frequency in Mouse Bone Marrow by Intraperitoneal Injection(i.p.)

目的：寻找一次染毒条件下,腹腔注射阿霉素诱导小鼠骨髓细胞微核率出现峰值的时间-剂量条件。方法：将150只小鼠随机分为5组,每组30只,雌雄各半。腹腔注射,1次给药,对照组给生理盐水,处理组的阿霉素剂量分别是2.5 mg/kg、5 mg/kg、10 mg/kg和20 mg/kg。染毒18 h、24 h、30 h、48 h和72 h后取小鼠骨髓进行微核分析,评价阿霉素对小鼠的遗传毒性作用。结果：理想的腹腔注射诱导的小鼠骨髓细胞微核率峰值的条件就是：一次染毒,阿霉素剂量在10%LD50即2.5 mg/kg至80%LD50即20 mg/kg的范围内剂量越大微核率越高,取样时间在30~48 h间微核率最高。

Objective to study time-dose effect of doxorubicin on inducing the micronueleus frequency in mouse bone marrow by intraperitoneal injection once. Methods 150 mice are divided into 5 groups randomly, 30 mice one group, half male and half female. Each mouse is injected once by intraperitoneal injection. The dose is： control group is injected pHysiological saline; the dose of the remaining four groups is 2. 5 mg/kg, 5 mg/kg, 10 mg/kg, 20 mg/kg respectively. Micronucleus test were performed in mice after 18 h, 24 h, 30 h, 48 h or 72 h, and the micronucleus frequency in mouse bone marrow and estimating doxorubicin genotoxicity in mice were counted. Results In the range of 2. 5 -20 mg/kg, with the increasing of dose, the percentage of micronucleus is higher, and the opti- mal sampling time is 48h after treatment.