高效表达AIM工程菌的发酵培养基及发酵条件优化

Optimization of Fermentation Mediums and Fermentation Conditions for AIM Production by Engineering Bacteria

为了进一步提高工程菌的载脂蛋白AI米兰突变体(AIM)的表达量,降低发酵成本,通过碳氮源优化实验并结合正交实验筛选出该工程菌产AIM的最适培养基为(g/L):蔗糖10,酵母粉10,硫酸铵6和NaCl 10;最适发酵条件为:装液量30 mL,接种量8%,诱导时机A600 1.0,诱导剂浓度0.8 mmol/L,诱导时间6 h.在此优化的条件下,AIM的表达量为2.26 g/L,是未优化条件下的1.58倍.

The optimal medium and optimal fermentation condition for AIM production by engineering bacteria were founded through carbon and nitrogen sources optimization experiments and orthogonal experiments. The optimal medium （g/L） ：sucrose 10,yeast powder 10, ammonium sulfate 6 and NaCl 10. The optimal fermentation condition ：liquid loading 30 mL,inoculum size 8%,induction opportunity A600 1.0,inductor concentration 0.8 mmol/L,induction time 6 h. Expression level of AIM is 2.26 g/L in the optimized conditions, which is the 1.58 times of the expression level in the basic conditions.