大鼠慢性高眼压下视网膜损伤中HIF-1α和caspase-9的作用

Role of HIF-1α and caspase-9 signal in rat retinal injure on chronic high ocular pressure

目的:探讨HIF-1α和caspase-9表达与高眼压视网膜损伤的关系。方法:大鼠60只随机分为6组,每组10只20眼。分别为假手术对照组;高眼压3,7,14,21,28d组。巩膜静脉烧烙法制作高眼压模型。应用免疫组织化学法,RT-PCR法,Western印迹法检测各组视网膜组织中HIF-1α和caspase-9基因mRNA及蛋白表达。结果:HIF-1α和casepase-9阳性染色主要位于视网膜内层即视网膜节细胞层和内颗粒层。HIF-1α和caspase-9mRNA和蛋白在正常视网膜中有低浓度的表达,高眼压3d后,表达明显上升,HIF-1α到7d时达到高峰,caspase-9到14d达到高峰,随后有所下降,但仍明显高于正常组的表达水平,差异有统计学意义。结论:HIF-1α和caspase-9是青光眼神经节细胞凋亡的发生和进展中重要的病理生理机制。

AIM： To research the mRNA and protein expressions of hypoxia-inducible factor-It（ （HIF-lcx） and caspase-9 in normal retina and ocular hypertention retina, investigate the relation of glaucomatous retinal degeneration and HIF-1α and caspase-9 signal transduction Path. METHODS： Coagulating two episcleral veins unilaterally in rats with electric coagulator to establish the glaucoma model. Use immunochemistry, PT-PCR and Western Blot analysis to examine the expression of HIF-la and caspase-9 genes. RESULTS： The expression of HIF-1α and caspase-9 were very faint immunostaining in normal retina. In the glaucomatous retina, immunostaining for HIF-1α the inner nuclear. In RT-PCR and Western Blot, HIF-la and caspase-9 genes mRNA and protein were faintly expressed in normal rat retina, and the expression of them increased in lOP elevation model rats with the time going on. The peak expression of HIF-1α was on the 7th day after lOP elevated. The peak expression of caspase-9 was on the 14th day then decreased but still maintained at a higher level. The expression among experimental groups was statisticlly different compared with the control group. CONCLUSION： HIF-la and caspase-9 participates in signal transduction of retinal neurons apoptosis during retinal injury on chronic high ocular pressure.