黄芩苷对家兔视网膜炎性水肿厚度影响的研究

Research of baicalin's effect on the thickness of inflammation-mediated retinal edema in rabbit

目的:研究黄芩苷对炎性视网膜水肿的影响。方法:建立炎性视网膜水肿的动物模型,用OCT测量黄芩苷对炎性视网膜水肿过程中视网膜厚度的影响。新西兰白兔15只(30个视网膜)随机分为5组,建立炎性视网膜水肿模型后,组1注射黄芩苷溶液(160mg/L,20mL/kg),2次/d;组2使用黄芩苷溶液(40mg/L,20mL/kg),2次/d;组3使用黄芩苷溶液(10mg/L,20mL/kg),2次/d;组4使用球后注射曲安奈德,给药剂量为20mg(0.5mL)。组5为对照组,被耳缘静脉注射等量BSS。分别在建模后1,2,4,6,10,14,21d用光学相干断层扫描(opitical coherence tomograph,OCT)测量视网膜的平均厚度。结果:与炎性对照组相比,黄芩苷治疗组视网膜厚度明显降低,两组差异具有统计学意义(P<0.01);黄芩苷组与激素组比较,48h内有差别,但差异不显著(t=5.85,4.3;0.001<P<0.01);2d后,两组差异性消失(t<2.447,P>0.05),从统计学角度看两者无差别。结论:黄芩苷能够有效抑制炎性视网膜细胞外水肿,并且与皮质激素具有相同的药效,并且无明显毒副作用。

AIM： To establish the inflammatory retinal edema animal model and research the influence of the baicalin on the inflammatory retinal edema. METHODS： Opitical coherence tomograph （OCT） was used to survey the effect of baicalin on the retina thickness in inflammatory retinal edema. 15 inflammation- mediated rabbits were randomly assigned to five groups, each group included 3 rabbits（6 retinas）, which received different treatments, respectively： baicalin （ 160mg/L, 20mL/kg） by iv. administration bid; baicalin （40mg/L, 20mL/kg） by iv. administration bid; baicalin （10rag/L, 20mL/kg） by iv. administration bid; triamcinolone acetonide（TA） retrobulbar injection of 20mg respectively; the control group was injected physiologic saline in the same volume. The effect was measured by the thickness of retinas of OCT on day 1, 2, 4, 6, 10, 14 and 21 respectively.RESULTS： Compared with the inflammatory control group, the thickness of baicalin group reduced apparently, the difference was remarkable（ P〈 0.01 ）, and the difference between baicalin group and TA group after 2 days were not statistically significant（ t 〈 2.447, P 〉 0.05）. CONCLUSION： The baicalin could suppress the inflammatory retina cell edema effectively, it even has the same drug efficacy with the TA, and no obvious poisonous side effect.