国内外甜高粱种质遗传多样性的SSR分析

Genetic Diversity of Domestic and Foreign Sweet Sorghum Germplasm Revealed by SSR Markers

用24对扩增带型稳定的SSR引物,研究了206份国内外甜高粱种质资源的遗传变异,共检测出220个等位基因,每对引物检测出2～19个等位基因。引物位点的多态信息含量指数(PIC)变幅在0.50～0.87。利用220个多态性标记计算206份甜高粱品种之间的遗传相似系数(GS),范围在0.32～0.96之间。利用SSR标记遗传相似系数矩阵,按UPGMA方法对206份甜高粱品种进行聚类分析,将其分为A、B两大组,B组只包含3个品种,与其他品种遗传关系较远,A组进一步被分成15个类群,农艺性状近似的聚在同一类群。

Simple sequence repeats（SSR） markers were adopted in heterotic grouping of 206 sweet sorghum germplasms.Twenty-four SSR primers giving stable amplified profiles detected 220 alleles among the lines tested.The average number of alleles per SSR locus was 8.19 with arrange from 2 to 19.The value of polymorphism information content（PIC） for each SSR locus varied from 0.50 to 0.87 with an average of 0.76.The genetic similarity coefficients（GS） between 206 sweet sorghum varieties were calculated using 220 alleles,ranging from 0.32 to 0.96,with an average 0.69.Cluster analysis was done for 206 accessions using the genetic similarity matrix by way of UPGMA.They were divided into two groups A,B.Group A was further divided into 15 subgroups.