水稻子粒硒含量的遗传及QTL检测

Inheritance of Selenium Contents and QTL Detection in Rice Grains

富硒功能水稻具有富硒、优质、保健、防病、安全等特点,已成为当前研究的热点。子粒硒含量的QTL定位对研究富硒功能水稻的遗传育种具有重要的意义。以籼稻亲本奉新红米和明恢100杂交的145个株系的F2群体构建遗传连锁图谱,图谱拟合92个SSR标记位点,覆盖水稻基因组2187.5 cM,标记间平均遗传距离为23.7 cM,占水稻全基因组的49.2%。采用复合区间作图法,对水稻子粒硒含量进行QTL分析,在第5染色体上共检测到2个新的水稻子粒硒的QTL,对表型变异的贡献率分别为6.39%、8.01%。

Enriched-selenium rice that is characterized with high content of selenium,high quality,health care,disease prevention,security and so on,has became one hot issue in scientific research.QTL location for selenium contents in rice grains is of great significance to the study of selenium-rich rice genetics and breeding in selenium breeding program.The genetic linkage map including 92 SSR markers was constructed with a F2 population consists of 145 lines from a cross of Oryza sativa indica Fengxinhongmi and Minghui 100,and the quantitative traits loci（QTL）analysis on selenium content in rice grains was done by using composite interval graphic method.By composite interval mapping method of software windows QTL Cartographer 2.5,it was determined that two selenium content QTLs qSe-1 and qSe-2 located in the fifth chromosome,the contribution rate was 6.39% and 8.01% respectively.