转不同rol基因741杨株系相关性状比较分析

Comparison of Related Traits among the Transgenic Poplar 741 with Different rol Gene

对已转入rolB、rolC基因741杨株系进行分子检测,并对其根系生长状况、苗高、叶绿素a和b、叶绿素荧光以及内源激素等指标进行测定。结果表明,转rolB、rolC基因741杨各株系发根率、发根量均高于未转基因对照。转rolB基因741杨株系苗高与对照无显著差异,叶绿素a、b含量上升,Fv/Fm、PI值下降,IAA、ZT含量上升,ABA含量下降。转rolC基因741杨各株系顶端优势减弱,植株矮化,叶绿素a、b含量降低,Fv/Fm、PI值下降,IAA、ZT、ABA含量上升。总体比较,转rolB基因741杨株系生根率,叶绿素a、b含量,PI值、ZT和IAA含量均高于转rolC基因741杨株系。

In this study,the transgenic poplar 741 with rolB and rolC were detected for molecule and their root growth variation,height,chlorophyll a,b,chlorophyll fluorescence characteristics,as well as endogenous hormones were determined.The results showed that the root number and rooting rate of transgenic poplar 741 with rolB and rolC were higher than the control.The transgenic poplar 741with rolB height with no significant difference between the control,chlorophyll a,b content increased,Fv/Fm,PI values decreased,IAA,ZT content increased,ABA content decreased.The transgenic poplar 741with rolB and rolC： the top edge of the weakened,dwarf plants,chlorophyll a,b content was reduced,Fv/Fm,PI values decrease,IAA,ZT,ABA content increased.Overall comparison,the transgenic poplar 741with rolB rooting rate,chlorophyll a,b content,PI value,ZT and IAA levels were higher than the transgenic poplar 741with rolC.