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HSF1对氧化应激诱导内皮细胞凋亡的保护作用的机制研究 被引量:2

Protective Role of Heat Shock Transcription Factor 1 on Apoptosis of Cardiac Microvascular Endothelial Cell During Oxidative Stress
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摘要 目的探讨热休克转录因子1(HSF1)对过氧化氢(H2O2)刺激后心脏微血管内皮细胞内活性氧簇(ROS)水平和细胞凋亡的影响。方法体外培养大鼠心脏微血管内皮细胞,分别单独转染HSF1质粒、凋亡信号调节激酶1(ASK1)质粒或共转染HSF1及ASK1质粒。转染48h后用1mmol/LH2O2刺激细胞30min,检测并比较各组细胞凋亡情况和胞内ROS水平。结果 (1)H2O2刺激后,各组细胞凋亡较刺激前明显增加(P<0.05);且在相同刺激条件下,HSF1转染组细胞凋亡明显少于对照组(P<0.05),ASK1转染组明显多于对照组(P<0.05),而共转染组与ASK1转染组相比明显减少(P<0.05)。(2)H2O2刺激后,各组细胞内ROS水平较刺激前都显著升高(P<0.05);且在相同刺激条件下,各组细胞内ROS水平较刺激前ROS水平增高的幅度:HSF1转染组明显低于对照组(P<0.05),ASK1转染组和共转染组与对照组无明显差异,而共转染组与HSF1转染组相比有升高趋势,与ASK1转染组相比有降低趋势。结论 HSF1可以通过抑制细胞内ROS产生对抗氧化应激诱导的细胞凋亡,保护心脏微血管内皮细胞。ASK1可能干扰HSF1的保护作用。 Objective To explore the effects of heat shock transcription factor 1(HSF1) on the levels of reactive oxygen species (ROS) and apoptosis in H2O2-induced rat cardiac microvascular endothelial cells. Methods Rat cardiac microvascular endothelial cells were cultured and transfected with the plasmids of HSF1,apoptosis signal-regulating kinase 1(ASK1) or HSF1 with ASK1.Then cells were stimulated with or withou 1 mmol/L H2O2 for 30 minutes. 48 hours later, apoptosis was assessed by TUNEL and ROS levels were measured by flow cytometer. Results (1) Treated with H2O2, apoptosis of every group raised signifi cantly (P0.05). And in the H2O2-stimulated cells, apoptosis in cells transfection with HSF1 signifi cantly reduced as compared with control(P0.05), and it signifi cantly increased in ASK1 group when compared with control(P0.05) Meanwhile, apoptosis in the cells transfected with both HSF1 and ASK1 significantly reduced when compared with group transfected with ASK1(P0.05). (2) Treated with H2O2,ROS levels of every group raised signifi cantly (P0.05).And in the H2O2-stimulated cells, ROS levels after transfection with HSF1 increased less than group without transfection(P0.05),while ROS levels in the cells transfected with both HSF1 and ASK1 was slightly increased more than group transfected with HSF1 alone and less than group transfected with ASK1 alone. Conclusion HSF1 can protec cardiac microvascular endothelial cells from oxidative stress through down-regulation of ROS levels and apoptosis in the cells. While ASK1 maybe weaken the protective action.
出处 《中国分子心脏病学杂志》 CAS 2010年第3期162-166,共5页 Molecular Cardiology of China
基金 国家自然科学基金(30570741) 2008年杨森科学研究基金(Janssen Research Council China)
关键词 热休克转录因子1 凋亡信号调节激酶1 活性氧簇 细胞凋亡 微血管内皮细胞 HSF1 ASK1 ROS Apoptosis Microvascular endothelial cells
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参考文献20

  • 1Chrisrians ES,Yan LJ,Benjamin IJ.Heat shock factor 1 and heat shock Proteins:critical parteners in protection against acute cell injury.Crit Care Med,2002,30(1):S43-S50.
  • 2Santos-Marques J,Carvalho F,Sousa C,et al.Cytotoxicity and cell signaling induced by continuous mild hyperthermia in freshly isolatedmouse hepatocytes.Toxicology,2006,224(3):210-218.
  • 3Nishizawa J,Nakai A,Matsuda K,et al.Reactive oxygen species play an important role in the activation of heat shock factor 1 in ischemicreperfused heart Circulation,1999,99(7):934-941.
  • 4Gotoh Y,Cooper JA.Reactive oxygen species-and dimerization-induced activation of apoptosis signal-regulating kinase 1 in tumor necrosis factor-alpha signal transduction.J Biol Chem,1998,273(28):17477-17482.
  • 5Knowlton AA,Kapadia S,Tone-Amione G,et al.Differential expression of heat shock proteins in normal and failing human hearts.J Mol Cell Cardiol,1998,30(4):811-818.
  • 6He XR,Liu YM,Sharma V,et al.ASK1 associates with troponin T and induce troponin T phosphorylation and contractile dysfunction in cardiomyocytes.American Journal of Pathology,2003,163:243-251.
  • 7何靖康,顾振纶,秦正红,马海涛,秦涌,谢燕.PGA1对缺氧再给氧大鼠心脏微血管内皮细胞ICAM-1表达的影响[J].中国药理学通报,2005,21(2):194-197. 被引量:6
  • 8Manalo DJ,Liu AY.Resolution,detection,and characterization of redox conformers of human HSF1.J Biol Chem,2001,276(26):23554-23561.
  • 9JacquierSarlin MR,Polla BS.Dual regulation of heat-shock transcription factor(HSF)activation and DNA-binding activity by H2O2:role of thioredoxin.Biochem J,1996,318:187-193.
  • 10Murapa P,Gandhapudi S,Skaggs HS,et al.Physioligical fever temperature induces a protective stress response in T lymphocytes mediated by heat shock factor-1(HSF1).Journal of immunology,2007,179(12):8305-8312.

二级参考文献9

  • 1Albert S,Baldwin Jr. The NF-κB and IκB proteins:new discoveries and insights[J].Am Rev lmmunol,1996,14(2):649-51.
  • 2Boyle EM Jr, Pohlman TH, Cornejo CJ et al.Endothelial cell injury in cardiovascular surgery:Ischemia-reperfusion[J]. Ann Thorac Surg,1996,62(6):1868-75.
  • 3Williams FM. Neutrophils and myocardio reperfusion injury[J].Pharmacol Ther,1996,72(1):1-12.
  • 4Nishida M,Carley WW, Gerritsen ME et al.Isolation and characterization of human and rat cardiac microvascular endothelial cells[J].Am J Physiol,1993,264(2):H639.
  • 5Shreiber E,Mathias P, Muller MM et al.Rapid detection of octamer binding proteins with prepared from a small number of cells[J].Nucleic Acids Res,1989,17(3):6419-25.
  • 6Edward M, Boyle Jr, Timothy G. Treating myocardial ischemia-reperfusion injury by targeting endothelia cell transcription[J]. Ann Thorac Surg,1999,68(5):1949-53.
  • 7Rossi A,Elia G,Santoro MG.Inhibition of nuclear factor kappa B by prostaglandin A1:An effect associated with heat shock transcription factor activation[J].Proc Natl Acad Sci USA,1997,94(2):746-50.
  • 8Sicbenlist U,Franzoso G,Brown K.Structure, regulation,and function of NF-κB[J].Ann Rev Cell Biol,1994,10(3):405-55.
  • 9王爱忠.血管内皮细胞的保护[J].国外医学(麻醉学与复苏分册),1997,18(1):22-24. 被引量:4

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同被引文献26

  • 1高培国,强辉,凌鸣.葛根素对过氧化氢诱导的血管内皮细胞损伤的保护作用[J].西安交通大学学报(医学版),2012,33(2):245-248. 被引量:45
  • 2钱睿哲,张国平,金惠铭,王文健,乐飞,史连国,曲晓义.大花红天素对脑微血管内皮细胞凋亡的双相调节作用及机制(英文)[J].中国病理生理杂志,2005,21(11):2086-2090. 被引量:6
  • 3沈明花,金成俊.榆干离褶伞溶栓酶的纯化及酶学性质研究[J].食品与发酵工业,2007,33(8):45-47. 被引量:18
  • 4ROSS R. Atherosclerosis-an inflammatory disease[J]. New EnglandJournal of Medicine, 1999, 340(2): 115-126. 001:10.1056/NEJM199901143400207.
  • 5SANDERSON T H, REYNOLDS C A, KUMAR R,et al. Molecularmechanisms of ischemia-reperfusion injury in brain: pivotal roleof the mitochondrial membrane potential in reactive oxygenspecies generation[J]. Molecular Neurobiology, 2013, 47(1): 9-23.DOI:10.1007/sl2035-012-8344-z.
  • 6LIU S Y, CHEN C L, YANG T T, et al. Albumin prevents reactiveoxygen species-induced mitochondrial damage, autophagy, andapoptosis during serum starvation[J]. Apoptosis, 2012,17(11):1156-1169. DOI:10.1007/sl0495-012-0758-6.
  • 7LIANG D, ZHOU Q, ZHANG J, et al. A novel chenodeoxycholicacid-verticinone ester induces apoptosis and cell cycle arrest inHepG2 cells[J]. Steroids,2012,77(13): 1381-1390. D01:10.1016/j.steroids.2012.08.013.
  • 8FULDA S. Cell death in hematological tumors[J]. Apoptosis, 2009,14(4): 409-423. DOI:10.1007/sl0495-008-0306-6.
  • 9ESKES R, DESAGHER S, ANTONSSON B,et al. Bid induces theoligomerization and insertion of Bax into the outer mitochondrialmembrane[J]. Molecular and Cellular Biology, 2000’ 20(3): 929-35.D01:10.1128/mcb.20.3.929-935.2000.
  • 10吴萍,陈思,程文晋,江蓓蕾,张旭东,张林杰.线粒体凋亡途径参与TRAIL诱导胃腺癌细胞凋亡[J].安徽医科大学学报,2007,42(6):599-603. 被引量:5

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