多聚鸟氨酸海藻酸钡微囊物理性能和生物相容性研究

Physical Properties and Biocompatibility of Ba-Alginate-Poly-L-Ornithine-Alginate microcapsules

目的验证多聚鸟氨酸(PLO)与海藻酸钡(BPA)微囊结合制备的多聚鸟氨酸BPA(B-PLO-A)微囊是否能够提高传统BPA微囊的物理性能和生物相容性。方法用静电微囊发生仪制备多聚鸟氨酸海藻酸钡(B-PLO-A)微囊和多聚赖氨酸海藻酸钡(B-PLL-A)微囊,通过对微囊在低渗环境中的形态变化和体外培养后直径变化的观察,以及对微囊在机械振荡后破损率的计算来评价微囊的物理性能;然后将两种微囊移植入大鼠腹腔,观察两者的完整性和生物相容性。结果 B-PLO-A微囊较B-PLL-A微囊在低渗环境下更稳定坚固;体外培养14d时B-PLO-A微囊较B-PLL-A微囊直径增加的程度更小;机械振荡96h后,B-PLO-A微囊的完整率为(99.3±1.0)%,B-PLL-A微囊为(96.2±1.5)%;腹腔移植2、4和8周后,从大鼠腹腔内取出的B-PLO-A微囊与B-PLL-A微囊结构均较为完整,呈圆形,表面光滑,光镜观察无明显结缔组织包裹,移植8周后B-PLO-A微囊完整率为(97.3±2.1)%,B-PLL-A微囊完整率为(95.4±2.4)%。结论 B-PLO-A微囊的物理性能比B-PLL-A微囊有所提高,同时保持良好的生物相容性。

Objective To test and verify whether Ba-alginate-Poly-L-Ornithine-Alginate microcapsules（B-PLO-A） can improve the physical properties and biocompatibility of the traditional BPA microcapsules.Methods The B-PLO-A and Ba-alginate-Poly-L-lysine-alginate（B-PLL-A） microcapsules were made by the static generator.The physical property of the microcapsules was evaluated by observing the morphological changes of the microcapsules in the hypotonic environment,changes in diameter of microcapsules in vitro culture and calculating broken microcapsules ratio by shaking method.The biocompatibility was observed by transplanting into peritoneal cavity of rat.Results B-PLO-A microcapsules are stronger and more stable in a hypotonic environment than B-PLL-A microcapsules.After 96 h mechanism shaking,the unbroken microcapsules ratio of B-PLO-A and B-PLL-A microcapsules were （99.3±1.0）% and （96.2±1.5）% respectively.The microcapsules were retrieved from peritoneal cavity of rat at 2,4 and 8 weeks after transplantation,most of the microcapsules were of integrity,rotundity,and surface smooth without obviously bundled by connective tissue.8 weeks after transplantation the intact microcapsules ratio of B-PLO-A and B-PLL-A microcapsules were （97.3±2.1）% and （95.4±2.4）% respectively.Conclusion B-PLO-A microcapsules as a whole have bettermechanical strength compared with B-PLL-A microcapsules,while maintaining a good biocompatibility.