血管抑素对角膜新生血管及白介素-1α表达的作用

EFFECT OF ANGIOSTATIN ON RAT CORNEAL NEOVASCULARIZATION AND THE EXPRESSION OF INTERLEUKIN-1α

目的:观察血管抑素(angiostatin,AS)对鼠碱烧伤角膜新生血管(corneal neovascularization,CNV)及白介素-1α(IL-1α)表达的作用,探讨AS对CNV的作用及机制。方法:105只SD大鼠随机取5只做正常对照,其余100只制作左眼碱烧伤模型,随机分为4组(每组25只),分别予生理盐水(A组)、0.1%地塞米松眼液(B组)、10μg/mLAS液(C组)、20μg/mLAS液(D组)滴眼,于碱烧伤后1、3、7、14、21d每组随机取5只大鼠麻醉后裂隙灯显微镜下观察,计算各组CNV面积,处死大鼠取角膜,行病理组织检查及酶链免疫吸附实验测定各组IL-1α的表达。结果:C组、D组碱烧伤后炎症细胞浸润减少,碱烧伤后第3天起各时间点CNV面积均明显小于A组(P<0.05)。碱烧伤后各时间点C组、D组IL-1α表达量均明显少于A组(P<0.05)。结论:AS能显著抑制碱烧伤CNV,可能与其抑制IL-1α的表达,抑制炎症反应有关。

Objective： To investigate the effect of angiostatin on rat corneal neovascularization and the ex pression of interleukin-1α after alkali burned, and to elucidate the mechanism of angiostatin. Methods：Cornea models were established in 105 SD rats, which were randomly divided into normal group（ n = 5） and experimental group（ n = 100, induced by alkali burn）, then were randomly divided into four groups： group A,B,C and D. Slit-lamp microscope was performed and the area of corneal neovaseularization was calculated at 3,7,14,21days after injury. Then 5 rats were randomly sacrificed, the cornea was taken for histopathological test and IL-1α ELISA assay. Results： The infiltrated inflammatory cells and the areas of corneal neovascuIarization in groups C and D were less than those in group A after injury（ P 〈0.05）. The expression of IL-1α was statistically significant difference between groups C,D and A（ P 〈0.05）. Conclusion： Angiostatin can effectively inhibit rat corneal neovaseularization after alkali burned. The probably mecha- nism was down-regulation the expression of IL-1α and reduce inflammatory response.