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罗格列酮对人肾小管上皮细胞转分化的影响

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摘要 目的:观察罗格列酮(rosiglitazone,RGZ)对TGF-β1刺激人肾小管上皮细胞转分化的影响。方法:人肾小管上皮细胞(HK-2)经同步培养后分为3组:正常对照组,TGF-β1刺激组,TGF-β1+RGZ组(10μmol/LRGZ)。给予TGF-β1刺激24h后,分别应用WesternBlot和免疫荧光方法检测上皮细胞转分化相关指标的表达变化;应用Transwellassay检测细胞迁移能力。结果:①与正常对照组比较,TGF-β1刺激组细胞呈梭形变,E-钙黏素蛋白表达减弱(P<0.01),波形蛋白、纤维连接蛋白表达增强(P<0.01);细胞的迁移能力亦明显增强。②与TGF-β1刺激组比较,TGF-β1+RGZ组细胞恢复为多边形或圆形,E-钙黏素蛋白表达增加(P<0.01),波形蛋白、纤维连接蛋白表达减弱(P<0.01);细胞的迁移能力亦明显减弱。结论:①TGF-β1刺激后HK-2细胞出现表型转化。②RGZ能够减弱TGF-β1刺激下HK-2细胞的表型转化。
作者 伍巧源 廖蕴华 杨桢华 霍冬梅 王成玉
机构地区 广西医科大学第一附属医院肾内科
出处 《广西医科大学学报》 CAS 2010年第3期370-373,共4页 Journal of Guangxi Medical University
关键词 罗格列酮 上皮细胞-间充质细胞转分化 转化生长因子-Β1
分类号 R692.5 [医药卫生—泌尿科学]
  • 相关文献

参考文献9

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二级参考文献21

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广西医科大学学报
2010年 第3期

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