波形蛋白与层粘连蛋白在人胚早期神经管中的表达

Study on the Expression of Laminin and Vimetin during the Period of Neural Tube Development of Human embryo

目的观察层粘连蛋白(LN)和波形蛋白(Vim)在人胚早期神经管发育中的表达变化,探讨人胚神经管发育早期细胞微环境的特点。方法收集早期人胚23天和45天标本8例,分别用LN和Vim抗体对人胚标本的组织切片行免疫组织化学ABC法染色。图像分析不同观测指标阳性细胞的积分光密度值,结果用t检验进行统计分析。结果人胚发育早期,LN和Vim在神经管的神经上皮层、中间层和边缘层的神经细胞胞中表达。在神经管发育到23天时,LN的积分光密度值(1258.17±635)比Vim的积分光密度值(2611.34±502)低(P<0.05)。结论在人胚早期,神经管的细胞基质成分以Vim为主,LN为辅,可能对神经干细胞的发育有重要作用。

Objective To investigate the expression and distribution of laminin and vimetin during the period of neural tube development of human embryo. Method Samples of early development of human embryo about 23 and 45 gestational days were collected and examined by using immunocytochemical ABC staining. Results There were lami- nin and vimetin immuno-positive substances in nerve cells of the epithelial lining and intermediate, marginal zones of neural tube. The value of integrated optical density of laminin-immunorecation during 23-days development of neural tube was lower than that of vimetin-immunorecation（1258.17± 635 vs 2611.34±502,P〈0.05）. Conclusion These results suggest that vimetin is main component in neural tube of early development of human embryo, while laminin being secondary, which may be of important role to the development of neural stem cells.