果胶酶高产菌的筛选及发酵条件的优化

Screening of pectinase producing strains and optimizing of its fermentation condition

采用平板分离法从腐烂的大枣中筛选出果胶酶的高产菌株,通过L16(45)正交试验对该菌种的深层液体发酵条件进行优化.结果表明:该菌种深层液体发酵最适的发酵条件为葡萄糖添加量1%,蛋白胨0.5%,吐温-60添加量0.03%,发酵培养基初始pH值4.5,发酵温度40℃,在此条件下菌株的产酶能力可以达到10758U·mL-1.

A strain producing pectinase highly was screened through plate method from rotten jujube. The strain was identified as a bacterial of G-. A L16 （45） design was used to optimize the liquid submerged fermentation condition. The results showed that the optimum conditions were as carbon source at 1 %, nitrogen source at 0.5 %,surfactant 0.03 %, intial pH 4.5 and temperature 40 ℃. The activity of pectinase produced by the strain under the optimum condition reached 10 758 U·mL^-1.