花生组培苗嫁接技术的研究

Study on Grafting Technique of Regenerated Plantlet in Peanut

为解决花生组培再生苗及转基因苗驯化移栽成活率低的难题,本试验以花生实生苗作为砧木,对花生嫁接技术进行了研究。试验结果表明:超净台内无菌嫁接效果较好,以再生苗或实生苗为接穗进行无菌嫁接,其成活率均达90%以上,明显高于室内嫁接成活率(71%～72%);无菌嫁接时12～15d苗龄的砧木效果最好;将无菌嫁接苗在培养基中培养3～5d后进行驯化移栽为最佳时期;不同花生基因型嫁接苗成活率在87%～95%;嫁接苗移栽田间后,其成活率高达94%,且100%结果。

In this paper,the grafting technique of peanut was studied by using germinated seedlings as the stocks. The result showed that the sterile grafting in clean bench using either seedlings or regenerated plantlets as the scion gave higher survival rate （up to 90% ） than that grafting in natural conditions （71% ～ 72% ）. Seedlings of 12 ～ 15 days after sowing in medium were the optimal stock. The grafted plantlets cultured on medium for 3 ～ 5 days were suitable for transplant to pots. The survival rates of different genotypes ranged from 87% to 95% . When the grafted plantlets were transplanted from pots to field,the survival rate was reached to 94% . One hundred percent of survived plants fructified in field.