导入反义Wx基因对珍汕97A及其F_1稻米品质的影响

Effects of Introducing Antisense Wx Gene on Grain Quality of Rice Cultivar Zhenshan97A and Its F_1 Generations

利用转反义Wx基因珍汕97B阳性转化植株,通过单株选择、回交转育,育成转反义Wx基因珍汕97A——优质珍汕97A。优质珍汕97A与珍汕97A比较,直链淀粉含量从25.6%下降到9.3%,胶稠度从45 mm提高到86 mm,垩白度从23.5%下降到7.1%;利用优质珍汕97A配制的F1稻米品质具有同样效应。

Through single plant selection and successive backcross,the transgenic rice Zhenshan 97A with antisense Waxy（Wx） gene,called the fine-quality Zhenshan 97A,was bred from the transgenic lines of Zhenshan 97B which positively expressed antisense Wx gene.Compared with non-transgenic Zhenshan 97A,the amylose content in grains of the transgenic Zhenshan 97A（fine-quality Zhenshan 97A） decreased by 16.3%,and the chalkiness degree decreased by 16.4%,while the gel consistency increased by 41mm.Its hybrid F1 generations had the same effect on rice quality as the fine-quality Zhenshan 97A.