香椿嫩叶中槲皮素和山奈酚的同板薄层鉴别及总黄酮的含量测定

TLC IDENTIFICATION OF QUERCETIN AND KAEMPFEROL AND DETERMINATION OF TOTAL FLAVONOIDS IN TOONA SINENSIS LEAVES

目的:建立香椿嫩叶的质量控制标准。方法:采用薄层色谱法对香椿嫩叶中槲皮素和山奈酚进行定性鉴别。采用UV法测定总黄酮。结果:在薄层色谱中能检测到槲皮素和山奈酚,总黄酮在0.025～0.25mg/ml范围内具有良好的线性关系(R=0.9998),回收率为98.30%,RSD为0.48%。结论:该方法操作简便、快速、有效、灵敏、准确、重现性好、专属性强,具有良好的重复性和回收率,可作为香椿嫩叶的质量控制方法。

Objective：To establish the quality control standards for Toona sinensis leaves. Methods： Quercetin and kaempferol were identified by TLC. The content of total flavonoids was detected by UV. Results： Quercetin and kaempferol could be detected by TLC. The calibration curve showed good linearity over the range of 0.025～0.25 mg/ml（R=0.9998）. The average recovery was 98.30% with RSD 0.48%.Conclusion：This method is simple,rapid,efficient,sensitive,accurate ,reproducibility and specificity are good,and with good repeatability and recovery,it can be used as a quantitative analysis method for this preparation.