实验性精索静脉曲张对大鼠睾丸结构和性激素影响

INFLUENCE OF EXPERIMENTAL VERICOCELE ON THE STRUCTURE OF TESTIS AND SERUM LEVELS OF SEX HORMONE IN RAT

目的观察实验性精索静脉曲张（EV）对大鼠睾丸结构及左精索静脉内促卵泡刺激素（FSH）、黄体生成素（LH）、睾酮（T）水平的影响。方法 40只雄性青春期Wistar大鼠随机分为EV 8周组、EV 12周组（每组12只）和相应对照组（各8只）,EV组部分结扎左肾静脉建立实验性大鼠EV模型,相应对照组接受假手术。分别于术后8周、12周处死动物,测量睾丸质量,光镜观察睾丸结构;放射免疫法测定曲张精索内静脉血和肾静脉血中FSH、LH、T的水平。结果 EV 12周、EV 8周组双侧睾丸质量与相应对照组比较明显下降（t=2.78~11.18,P〈0.05、0.01）,EV 12周组双侧睾丸质量较EV 8周组减轻（t=2.55、3.73,P〈0.05、0.01）。光镜下观察,EV 8周和EV 12周组大鼠双侧睾丸均有局灶性生精上皮退化,精子发生阻滞,生精细胞脱落和曲细精管萎缩等病理性改变。对照组、EV 8周组和EV 12周组大鼠左精索静脉血FSH和LH水平依次增加（t=3.53~9.51,P〈0.01）,后两者T水平较对照组降低（t=2.52、4.40,P〈0.05、0.01）。结论 EV可导致大鼠双侧睾丸质量下降,曲细精管病理性损害,性腺轴内分泌功能异常。

Objective To observe the impact of experimental varicocele（EV） on the structure of testis and the serum levels of follicle stimulating hormone（FSH）,luteinizing hormone（LH） and testosterone（T） in rats. Methods Forty male adolescent-age Wistar rats were randomly divided into EV-8-week group and EV-12-week group,with 12 rats in each group;and corresponding control groups,eight in each group.An EV model was created by partially ligating the left renal vein,and a sham operation was done for the controls.The animals were killed 8 and 12 weeks,respectively.The weight of the testis was measured,its structure observed light-microscopically,and the serum levels of FSH,LH and T of varicose vein blood and renal vein were determined by radioimmunoassay. Results Compared with those of the control,the weight of testis of EV groups was significantly lower（t=2.78-11.18;P0.05,0.01）.The weight of testis of rats in EV-12-goup were significantly lower than those of EV-8-week group（t=2.55,3.73;P0.05,0.01）.Light-microscopically,a focal degeneration of germinal epithelium,retardation of spermatogenesis and desquamation of spermatocytes were found in the testis of the EV rats.The serum levels of FSH and LH of the EV groups were significantly increased in order compared with those of the corresponding control groups（t=3.53-9.51,P0.01）,while the serum level of T decreased（t=2.52,4.40;P0.05,0.01）. Conclusion Experimental varicocele can lead to the decrease of the weight of bilateral testis,pathologic lesion,and pathocrinia of genitical gland.