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一株弗劳地枸橼酸杆菌中检出新ampC基因 被引量：2

A new ampC gene detected in Citrobacter freundii
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摘要 目的探讨1株耐亚胺培南弗劳地枸橼酸杆菌的耐药机制。方法采用浓度梯度法(Etest)检测对抗菌药物的最低抑菌浓度(MIC)。通过金属酶初筛试验(协同法)检测金属酶;改良Hoged试验检测碳青霉烯酶;头孢西丁三维试验检测AmpC酶;聚合酶链反应(PCR)检测耐药基因;DNA测序决定基因型;接合试验检测耐药基因的转移性。结果弗劳地枸橼酸杆菌临床分离株NC118对亚胺培南的MIC为>16μg/ml,金属酶初筛试验阴性,Hoged表型确证试验碳青霉烯酶阳性,AmpC酶阳性,PCR扩增及测序显示含有blaKPC-2、blaAmpC基因,该菌株所产AmpC酶基因与CMY-45型AmpC酶(GenBank:ACU00152.1)比较有5个氨基酸发生了改变,该blaCMY-2-like基因为一个新型的AmpC酶基因。结论在弗劳地枸橼酸杆菌中发现一种新的ampC基因(blaCMY-49)。 Objective To investigate the imipenem resistance mechanism of a clinical isolate of Citrobacter freundii NC118.Method The minimum inhibitory centrations（MICs） were determined by Etest.Double-disk synergy test was used for metallo-β-lactamase screening. Three dimention test was applied to identify AmpCs. Polymerase chain reaction（PCR） and DNA sequencing were carried out for determining antimicrobial resistance genes. Conjugation experiment was performed to determine whether resistance to carbapenem was likely transferred by plasmid.Result C. freundii strain NC118 was resistant to most of the clinically used antimicrobial agents.The isolate was negative for metallo-β-lactamase genes,but positive for blaKPC-2.The clinical isolate also produced AmpCs. A total of eleven antimicrobial-resistant genes were simultaneously detected in C. freundii strain NC118,including blaKPC-2,and blaCMY-49.Conclusion A new ampC gene,blaCMY-49,was discovered in Citrobacter freundii.
作者 胡龙华 熊建球 贾坤如 王小中 吕娇凤 胡晓彦 章白苓
机构地区 南昌大学第二附属医院
出处 《中国微生态学杂志》 CAS CSCD 2010年第6期527-530,共4页 Chinese Journal of Microecology
关键词 弗劳地枸橼酸杆菌 基因型 AMPC酶 KPC-2 Citrobacter freundii Genotype AmpC Carbapenemase KPC-2
分类号 R378 [医药卫生—病原生物学]
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参考文献18

  • 1LEE H K, PARK Y J, KIM J Y, et al. Prevalence of decreased susceptibility to carbapenems among Serratia marcescens, Enterobacter cloacae and Citrobacterfreundii and investigation of carbapenemases [ J ]. Diagn Microbiol Infect Dis,2005,52(4) :331-336.
  • 2RASHEED J K,BIDDLE J W,ANDERSON K F,et al. Detection of the Klebsiella pneumoniae carbapcncmase type 2 Carbapenem-hydrolyzing enzyme in clinical isolates of Citrobacter freundii and K. oxytoca carrying a common plasmid[ J]. J Clin Microbiol,2008 ,46(6) :2066-2069.
  • 3PEREZ-PEREZ F J, HANSON N D. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR [J]. J Clin Microbiol,2002,40(6) :2153-2162.
  • 4Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: 18th informational supplement (M100-S18). In: Wayne,PA: CLSI. 2008.
  • 5ARAKAWA Y, SHIBATA N, SHIBAYAMA K, et al. Cnvenient test for screening metallo-β-lactamase-producing gram-negative bacteria by using thiol compounds[ J]. J Clin Microbiol,2000,38( 1 ) :40-43.
  • 6COUDRON P E,MOLAND E S,THOMSON K S. Occurrence and detection of AmpC bcta-laclamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates at a Veterans Medical Center [J]. J Clin Microbiol,2000,38(5) :1791-1796.
  • 7NORDAMANN P, POIREL L. Carbapenemases in Gram-negative aerobes[ J]. Clin Microbiol Infect,2002,8 ( 8 ) :321-331.
  • 8YIGIT H, QUEENAN A M, ANDERSON G J, et al. Novel carbapenemhydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumonia [ J ]. Antimicrob Agents Chemother, 2008,52 (2) :809.
  • 9SMITH MOLAND E, HANSON N D, HERRER V L, et al. Plasmid-mediated carbapenem-hydrolysing β-lactarnase, KPC-2 ,in Klebsiella pneumoniae isolates [J ]. J Antimicrob Chemother,2003,51 ( 3 ) :711-714.
  • 10NAAS T, NORDMANN P, VEDEL G, et al. Plasmid-mediated carbapenem-hydrolyzing bcta-lactamasc KPC in a Klebsiella pneumoniae isolate from France [ J ]. Antimiemb Agents Chemother, 2005,49 ( 10 ) : 4423-4424.

二级参考文献51

  • 1张幸国,杜小幸,张嵘,魏泽庆,俞云松,陈亚岗,李兰娟.发现一株产KPC-2型碳青霉烯酶肺炎克雷伯菌[J].中华检验医学杂志,2006,29(9):824-826. 被引量：60
  • 2Yigit H, Queenan AM, Anderson GJ, et al. Novel carbapenemhydrolyzing beta-lactarnase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother, 2001,45:1151-1161.
  • 3Cai JC, Zhou HW, Zhang R, et al. Emergence of Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli Isolates possessing the plasmid-mediated carbapenem-hydrolyzing betalactamase KPC-2 in intensive care units of a Chinese hospital. Antimicrob Agents Chemother, 2008,52:2014-2018.
  • 4Villegas MV, Lolans K, Correa A, et al. First identification of Pseudomonas aeruginosa isolates producing a KPC-type carbapenem-hydrolyzing beta-lactamase. Antimicrob Agents Chemother, 2007,51 : 1553-1555.
  • 5Leavitt A, Navon-Venezia S, Chmelnitsky I, et al. Emergence of KPC-2 and KPC-3 in carbapenem-resistant Klebsiella Pneumoniae Strains in an Lsraeli hospital. Antimicrob Agents Chemother, 2007,51:3026-3029.
  • 6Naas T, Nordmann P, Vedel G, et al. Plasmid-mediated carbapenem-hydrolyzing beta-lactamase KPC in a Klebsiella pneumoniae isolate from France. Antimicrob Agents Chemother, 2005,49:4423-4424.
  • 7Cuzon G, Naas T, Demachy MC, et al. Plasmid-mediated carbapenem-hydrolyzing beta-lactamase KPC-2 in Klebsiella pneumoniae isolate from Greece. Antimicrob Agents Chemother, 2008,52:796-797.
  • 8Schlesinger J, Navon-Venezia S, Chmelnitsky I, et al. Extendedspectrum beta-lactamases among Enterobacter isolates obtained in Tel Aviv, Israel. Antimicrob Agents Chemother, 2005,49:1150- 1156.
  • 9Eckert C, Gautier V, Arlet G. DNA sequence analysis of the genetic environment of various blaCTX-M genes. J Antimicrob Chemother, 2006,57 : 14-23.
  • 10Anderson KF, Lonsway DR, Rasheed JK, et al. Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J Clin Microbiol, 2007,45:2723-2725.

共引文献106

同被引文献16

  • 1WALTHER-RASMUSSEN J,HΦIBY N.Class A carbapenemases. Journal of Antimicrobial Chemotherapy . 2007
  • 2HOSSIN A,FERRON MJ,PINO R M,et al.Plasmid-mediated carbap-enem-hydrolyzing enzyme KPC-2 in anEnterobactersp. Antimicrobial Agents and Chemotherapy . 2004
  • 3Queenan A M,Bush K.Carbapenemases:the versatileβ-lactamases. Clinical Microbiology Reviews . 2007
  • 4Clinical and Laboratory Standards Institute (CLSI).Perform-ance standards for antimicrobial susceptibility testing;nine-teenth informational supplement. M100-S19 . 2009
  • 5Villegas MV,Lolans K,Correa A, et al.First detection of the plasmid-mediated class A carbapenemase KPC-2 in clinical isolates of Klebsiella pneumoniae from South America. Antimicrobial Agents and Chemotherapy . 2006
  • 6Woodford N,Tierno PM Jr,Young K,et al.Outbreak ofKlebsiellapneumoniaeproducing a new carbapenem-hydrolyzing class A beta-lactmase,KPC-3,in a New York Medical Center. Antimicrobial Agents and Chemotherapy . 2004
  • 7Naas T,Nordmann P,Vedel G, et al.Plasmid-mediated carbapenem-hydrolyzing beta-lactamase KPC in a Klebsiella pneumoniae isolate from France. Antimicrobial Agents and Chemotherapy . 2005
  • 8Nordmann P,Poirel L.Emerging carbapenemases in Gram-negative aerobes. Journal of Clinical Microbiology . 2002
  • 9Cai JC,Zhou HW,Zhang R,et al.Emergence of Serratia marcescens, Klebsiella pneumoniae,and Escherichia coli isolates possessing the plasmid-mediated carbapenem-hydroiyzing beta-lactamase KPC-2 in intensive care units of a Chinese hospital. Antimicrobial Agents and Chemotherapy . 2008
  • 10陈慧红,沈波,王依满,俞云松,余素飞,戴冬玲,周君,傅鹰,陈佩珍,俞文娴.多重耐药鲍曼不动杆菌同源性分析[J].中国微生态学杂志,2008,20(1):65-67. 被引量：7

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中国微生态学杂志
2010年 第6期

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