应用肠杆菌科基因间重复序列分析多重耐药铜绿假单胞菌基因指纹图谱

Molecular epidemiological survey of highly multi-drug resistance Pseudomonas aeruginosa by Enterobacterial Repetitive Intergenic Consensus:an ERIC-PCR analysis

目的明确多重耐药铜绿假单胞菌在院内感染中的流行情况,为临床防治提供依据。方法用德灵MicroscanWalkAway96SI系统鉴定细菌和读取最小抑菌浓度。应用肠杆菌重复基因间隔共有序列(ERIC)-PCR对铜绿假单胞菌进行基因分型。结果多重耐药铜绿假单胞菌可扩增出丰富的区带,在药敏表型相似的情况下被分为9种基因型。结论流行于大连医科大学第一临床学院的多重耐药铜绿假单胞菌在各科室患者中呈现出散发的状态。ERIC-PCR指纹图谱基因技术分型方法简便快捷,便于铜绿假单胞菌医院感染流行病学监测。

Objective To verify the Molecular epidemiology of highly multi-drug resistant Pseudomonas aeruginosa and provide basis for the therapy and control of the nosocomical infection. Method Identification and the antibiotic susceptibility test were performed by Microscan WalkAway 96SI. The strains were typed by enterobacterial repetitive intergenicconsensus （ERIC）-PCR and then the electrophoresis in agarose gel was performed. Result ERIC-PCR genotyping method had high discrimination.23 strains of P.aeruginosa was identified as belong to 9 genotypes. Conclusion Highly multi-drug resistant P.aeruginosa has been sporadic;ERIC-PCR fingerprinting technique method can give faster and more reliable genotyping result,which serves as a tracking tool for nosocomial infection.