吡啶降解菌的生物降解特性

Biodegradation characterization of a pyridine-degrading strain

吡啶是一种含氮杂环化合物,是难降解的工业污染物。该文研究了纯菌株对吡啶的降解特性。从农药厂的污染土壤中分离到一株吡啶降解菌,经16SrRNA基因的部分序列比对分析,鉴定为副球菌属(Paracoccus sp.),命名为KT-5。考查了KT-5对吡啶的降解特性。结果表明:KT-5可以以吡啶为唯一碳源、能源和氮源生长,吡啶浓度达到1.331 g/L时,不会抑制KT-5的生长。在不同质量浓度的吡啶下KT-5的降解特性不同。当初始吡啶质量浓度低于0.9 g/L时,KT-5对吡啶的初始降解速率比最终降解速率高;当初始吡啶质量浓度高于0.9 g/L时,KT-5对吡啶的初始降解速率比最终降解速率低。

Pyridine is a intractable toxic organic and a N-heterocycle compound.The biodegradation characterization of pyridine was investigated in a pure culture using a pyridine-degrading strain isolated from the soil in a pesticide plant.The strain was identified as Paracoccus sp.on the basis of a partial 16SrRNA gene sequence analysis and designated as strain KT-5.Analysis of pyridine degradation indicated that KT-5 was able to utilize pyridine as the sole source of carbon,energy and nitrogen.High concentrations of pyridine（1.331 g/L） did not inhibit the growth of KT-5.KT-5 showed different degradation characteristics with different initial pyridine concentrations.When the pyridine concentration was below 900 mg/L,the initial KT-5 degradation rate was higher than the final degradation rate.When the pyridine concentration was above 0.9 g/L,the initial KT-5 degradation rate was lower than the final degradation rate.