烟夜蛾谷胱甘肽S-转移酶基因的克隆与时空表达分析

Molecular cloning and spatio-temporal expression analysis of glutathione S-transferase gene from Helicoverpa assulta(Guenée)

用RT-PCR方法,从烟夜蛾(Helicoverpa assulta(Guenée))成虫腹部克隆获得了1个谷胱甘肽S-转移酶(glutathione S-transferases,GSTs)基因的完整开放阅读框cDNA序列.该基因的开放阅读框全长636 bp,编码211个氨基酸残基,推测编码蛋白的分子量为24.2 kD,等电点为6.66.将该基因推导的氨基酸序列与其他物种的GSTs进行同源性和系统发育分析,发现该基因编码的蛋白属于昆虫特异性Epsilon家族成员,将该基因命名为HaGSTe2(GenBank登录号:GQ856239).HaGSTe2在雌、雄虫触角、喙、去除触角和喙的头、胸、腹、足和翅中均有表达,而且在卵、幼虫和蛹中也有表达.

The open reading frame cDNA of a novel glutathione S-transferase was cloned from Helicoverpa assulta,by using RT-PCR method.The results showed that the full length of open reading frame in this gene is 636 bp,encoding 211 amino acid residues,and the predicted molecular weight and isoelectric point are 24.2 kD and 6.66,respectively.Based on the sequence similarities and phylogenetic tree,the sequence obtained belongs to the epsilon family,hence named as HaGSTe2 and submitted to Genbank with accession No.GQ856239.HaGSTe2 transcript was clearly observed from the antennae of both male and female,proboscises,heads without antennae and proboscises,thorax,abdomen,legs and wings,and total developmental stages,including egg,larvae,pupae and adult.