气-质联用研究地锦草提取物对红色毛癣菌麦角甾醇的影响

Effect of extracts from Euphorbia humifusa on ergosterol in Trichophyton rubrum by GC-MS

目的:研究地锦草提取物对真菌细胞膜中麦角甾醇生物合成的影响。方法:红色毛癣菌经不同浓度的地锦草提取物作用后收集菌丝,经过漂洗、皂化、提取和N-甲基三甲基硅基三氟乙酰胺衍生化,采用气-质联用(GC-MS)法测定其麦角甾醇的含量。结果:地锦草提取物能使红色毛癣菌中的麦角甾醇含量下降(P<0.01),并有明显的剂量依赖性。结论:地锦草提取物是通过抑制真菌的麦角甾醇生物合成而发挥其抗真菌活性的。

OBJECrlVE To study the effect of Euphorbia humifusa extract on ergosterol biosynthesis in fungus. METHODS Rinse, saponification, extraction and silanization （MSTFA） of the nonsaponifiable lipids （NSLs） of Trichophyton rubrum were treated with varied concentrations of Euphorbia humifusa extract. Ergosterol in the NSLs was separated and its concentration as determined by the method of GC-MS. RESULTS Euphorbia humifusa extract inhibited ergosterol biosynthesis（P〈0. 01） with a dose-dependent manner in Trichophyton rubrum. CONCLUSION Euphorbia humifusa extract owes remarkable antifungal activity to its inhibitory effect on ergosterol biosynthesis in fungus.