泽泻提取物HPCE指纹图谱研究

Study on Fingerprint Spectrum of Rhizoma Alismatis Extract by HPCE

目的建立中药泽泻提取物毛细管电泳指纹图谱。方法采用毛细管区带电泳模式,压力进样:0.5Psi×10s,分离电压30kV,柱温25℃,DAD检测波长为202nm,带宽8nm,参比波长400nm,带宽20nm。运行缓冲液由0.1mol·L-1硼砂-0.1mol·L-1SDS-甲醇(4∶6∶2)构成,检测结果用相似度评价软件分析其指纹图谱的相似度。结果 10批次泽泻提取物毛细管电泳指纹图谱的相似度均在0.9以上,表明其指纹图谱整体面貌基本一致。结论所建立的泽泻提取物高效毛细管电泳指纹图谱稳定、可靠,可作为泽泻提取物的特征指纹图谱,该研究为泽泻提取物的定性鉴别及内在质量评价提供了新的参考依据。

OBJECTIVE To establish the fingerprint spectrum of Rhizoma Alismatis extract by HPCE.METHODS Using Capillary Zone Electrophoresis（CZE） patterns,injection pressure：0.5Psi×10 s,separation voltage 30kV,column temperature 25 ℃,DAD detection wavelength 202nm.Running buffer 0.1 mol·L^-1 borax-0.1 mol·L^-1 SDS-methanol（4：6：2）,and analysis the the similar by similar evaluated software.RESULTS All of the similarity are above 0.9,so the fingerprint integer feature of the 10 batches Rhizoma Alismatis extract are coincident essentially.CONCLUSION The established fingerprint spectrum is stable and credible,and could be used as characteristic fingerprint spectrum for Rhizoma Alismatis extract.And it will be a new reference for qualitation identification and quality evaluation of Rhizoma Alismatis extract.