异丙酚对大鼠前额叶皮层局部场电位的影响

Effect of propofol on local field potential of prefrontal cortex in rats

目的 评价异丙酚对大鼠前额叶皮层局部场电位的影响,以探讨其导致认知功能障碍的原因.方法 雄性SD大鼠30只,体重190～230 g,随机分为3组（n=10）：脂肪乳组（C组）、低剂量异丙酚组（P1组）和高剂量异丙酚组（P2组）,分别静脉输注10%脂肪乳0.01 ml·kg-1·min-1、异丙酚0.1 mg·kg-1·min-1和异丙酚0.5 mg·Kg-1·min-1 2h.给药后1 d进行改良Morris水迷宫实验,共测试5 d,记录逃避潜伏期、平台象限游泳时间、平台象限游泳路程占总游泳路程百分比和第1环点数.异丙酚给药后14 d,P1组和P2组分别静脉输注异丙酚0.1或0.5 mg·kg-1·min-1,给药1～2 h期间记录前额叶皮层局部场电位.结果 与C组和P1组比较,P2组逃避潜伏期延长,平台象限游泳时间缩短,平台象限游泳路程占总游泳路程百分比和第1环点数降低（P〈0.05）.P1组复杂度和功率谱高于P2组（P〈0.05）.结论 高剂量异丙酚可抑制大鼠前额叶皮层神经元放电,而导致认知功能障碍.

Objective To evaluate the effect of propofol on local field potential of prefrontal cortex in rats in order to investigate the reason why pmpefol leads to cognitive dysfunction.Methods Thirty healthy male SD rats weighing 190-230 g were randomly divided into 3 groups（n=10 each）：intralipid group（group C）,low dose propofol group（group P1）and high dose propofol group（group P2）.In group C,P1 and P2,10% intralipid 0.01 ml·kg-1·min-1,pmpofol 0.1 mg·kg-1·min-1,and propofol 0.5 mg·kg-1·min-1 were infused iv through the caudal vein for 2h respectively.The modified Morris water-maze （MWM） test was performed twice a day for 5 consecutive days one day after administration.The escape latency,swimming time in platform quadrant,percentage of swimming distance in platform quadrant in the total swimming distance and the fLrst central point were recorded.Propofol 0.1 and 0.5 mg·kg-1·min-1 were infused iv in group P1 and P2 respectively 14 days after propfol administration.Local field potential of prefrontal cortex was recorded at 1-2 h of administration.Results Compared with group C and P1,the escape latency was prolonged,the swimming time in platform quadrant was shortened,and the percentage of swimming distance in platform quadrant in the total swimming distance and the first central point were signifieandy decreased in group P2（P〈0.05）.The complexity and power spectrum were significantly lower in group P2 than in group P1（P〈0.05）.Conclusion The high dose of propofol Can inhibit prefrontal cortex neuronal discharge activity to result in cognitive dysfunction.