腺苷后处理对大鼠心肌缺血再灌注时血清IL-10和TNF-α浓度的影响

Effects of adenosine postconditioning on serum concentrations of IL-10 and TNF-α following myocardial ischemia-reperfusion in rats

目的 探讨腺苷后处理对大鼠心肌缺血再灌注时血清IL-10和TNF-α浓度的影响.方法 雄性SD大鼠24只,体重180～250 g,随机分为4组（n=6）：假手术组（S组）、缺血再灌注组（IR组）、缺血后处理组（IP组）和腺苷后处理组（AP组）.IR组、IP组和AP组结扎冠状动脉左前降支30 min后恢复再灌注.IP组缺血30 min时进行再灌注30 s缺血30 s,循环3次,然后再灌注120 min;AP组缺血30 min时静脉输注腺苷40 μg·kg-1·min-1,剂量1.5 mg/kg,然后再灌注120 min.于缺血前（基础状态）、缺血30 min、再灌注30、120 min时记录HR、SP和DP.再灌注120 min时采集动脉血样,测定血清IL-10和TNF-α的浓度.采集血样后,取心肌组织,测定MDA含量及心肌梗死面积,光镜下观察心肌病理学结果.结果 与S组比较,IR组HR、SP和DP降低,IP组和AP组SP降低,IR组、IP组和AP组血清IL-10、TNF-α浓度和心肌MDA含量升高,心肌梗死面积增加（P〈0.05）;与IR组比较,IP组和AP组HR、SP和DP升高,血清TNF-α浓度和心肌MDA含量降低,心肌梗死面积减小,血清IL-10浓度升高（P〈0.05）;IP组和AP组上述指标差异无统计学意义（P〉0.05）.IP组和AP组心肌病理学损伤程度轻于IR组.结论 腺苷后处理可促进IL-10生成,抑制TNF-α生成,从而减轻大鼠心肌缺血再灌注损伤.

Objective To investigate the effects of adenosine postconditioning （AP） on serum IL-10 and TNF-α concentrations following myocardial ischemia-reperfusion（VR）in rats.Methods Twenty-four SD ratsweighing 180-250 g were randomly divided into 4 groups（n=6 each）：group I sham operation （group S）;group Ⅱ myocardial I/R;group Ⅲ ischemic postconditioning（group IP）and group Ⅳ AP.Myocardial I/R was induced by 30 rain occlusion of anterior descending branch of left coronary artery followed by 120 min reperfnsion.IP was induced by 3 cycles of 30 s myocardial ischemia followed by 30 s reperfusion at the end of ischemia.In AP group adenosine 1.5 mg/kg was infused at 40μg·kg-1·min-1 before the onset of reperfusion.SP,DP and HR were recorded before ischemia （baseline） at 30 min of ischemia and 30 and 120 min of reperfusion.Arterial blood sarnples were collected at 120 min of repednsion for determination of serum TNF-α and IL-10 concentrations.The animals were then killed.Their hearts were removed for microscopic examination.Myocardial infarct size was measured and myocardial MDA content was determined.Results BP and HR were signilicandy decreased during reperfusion while myocardial infarct size.MDA content and serum concentrations of IL-10 and TNF-α were significantly increased in I/R group compared with group S.Ischemic and adenosine postconditioning significantly attenuated hypotension,reduced infarct size,myocardial MDA content and serum TNF-α concentration and increased serum IL-10 concentration in group AP and IP as compared with I/R group.There was no significant difference in the above changes between group AP and IP. Myocardial injury was ameliorated in group AP and IP as compared with I/R group. Conclusion Adenosine postconditioning can protect myocardium from I/R injury by increasing IL-10 production and inhibiting TNF-a release.