高原型藏绵羊KAP基因多态性与部分产毛性状的关系研究

Study of the Correlations between Polymorphism of KAP Gene and Wool Production Traits in Plateau Tibetan Sheep

旨在寻找KAP基因上与产毛性状相关的多态位点,为藏绵羊产毛性状的分子标记提供理论依据。利用PCR-SSCP技术研究了高原型藏绵羊KAP3.2、KAP7基因部分序列和KAP8基因外显子的多态性;采用最小二乘线性模型,分析了多态位点与毛长和产毛量性状的关联性;通过多态位点与经济性状的遗传相关预测了选择反应,确定了各性状的优势标记位点。研究表明:3个基因座都存在多态性,均检测到AA、AB和BB3种基因型。KAP3.2和KAP7基因AA型的毛长性状均显著高于BB型(P<0.05);KAP3.2基因AA型的产毛量性状显著高于AB型和BB型(P<0.05),KAP8基因AB型的产毛量性状显著高于AA型和BB型(P<0.05);毛长和产毛量性状上,KAP3.2基因的选择反应最大。KAP3.2可作为毛长和产毛量性状的优势标记基因。

This study was conducted to analyze the polymorphisms of KAP gene and aid to provide a theoretical foundation for molecular marker of wool production performance in tibetan sheep.Polymorphisms of partial sequence of KAP3.2 ,KAP7 and the exon of KAP8 were studied in plateau Tibetan sheep by PCR-SSCP,and the correlation between polymorphism of KAP and the traits of wool length and wool yield were analyzed using least square linear model.To determine the preponderant marker for every trait,the selection reaction was forecasted via thegenetic correlation between polymorphic loci and economic traits.The results showed that there were polymorphisms at three gene loci,and were detected three genotypes including AA,AB and BB.For the wool length trait,AA of KAP3.2 and KAP7 was significantly higher（P 0.05 ） than BB.For the wool yield trait,AA of KAP3.2 was significantly higher（P 0.05 ） than AB and BB,AB of KAP8 was significantly higher （P 0.05 ） than AA and BB.The selection reaction of KAP3.2 was higher than KAP7 and KAP8.KAP3.2 could be regarded as a preponderant marker for wool length and wool yield.