HPLC梯度洗脱法测定舒胸片中羟基红花黄色素A和阿魏酸的含量

Determiation of Hydrosafflower Yellow A and Ferulic Acid in Shuxiong Tablets by HPLC

目的:建立舒胸片(三七、川芎、红花)中羟基红花黄色素A、阿魏酸的含量HPLC测定方法。方法:用Agilent ODSC_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.12%磷酸溶液,梯度洗脱(0～17 min,25%甲醇;17～30 min,25%～40%甲醇);流速1 ml·min^(-1);波长分别为403 nm和320 nm;柱温35℃。结果:羟基红花黄色素A、阿魏酸得到很好分离,线性关系良好,羟基红花黄色素A、阿魏酸的平均加样回收率分别为100.0%,99.6%,RSD分别为1.19%,1.33%(n=5)。结论:本法结果准确,便于操作,可作为舒胸片的质量控制方法之一。

Objective： To establish a HPLC method for determination the hydrosafllower yellow A and ferulie acid in Shuxiong Tab- lets. Method： The Agilent ODS Cls colunm （250 mm ×4.6 mm,5 μm） was used,the methanol-0. 12% phosphoric acid solution as mo- bile phase ,gradient elution,the elution program was 0-17 min,25% methanol;17-30 min,25% -40% methanol,the flow rate was 1.0 ml. min-I. The delection wavelength was at 403 nm and 320 nm. Result： The linear ranges of hydrosafflower yellow A and ferulic acid were 0. 04-0. 41μg,r =0. 999 2,and 0. 03-0. 32 μg,r =0. 999 8. The average recoveries （n =5） were 100. 0% （RSD 1.19% ） ,99. 6% （RSD 1.33% ） respectively. Conclusion： The method is simple and accurate ,it suitable for quality control of Shuxiong Tablets.