小儿遗尿分散片中α-细辛醚、β-细辛醚的含量测定

Determination ofα-asarone and β-asarone in Children Yiniao Dispersible Tablets

目的:建立小儿遗尿分散片中α-细辛醚、β-细辛醚的含量测定方法。方法:采用反相高效液相色谱法。色谱柱:HypersilODS C_(18)柱(250 mm×4.6 mm,5μm);流动相:甲醇:水(60∶40);检测波长:257 nm;柱温:30℃;流速:1.0 ml·min^(-1)。结果:α-细辛醚在0.051～1.286 mg·ml^(-1)范围内与峰面积线性关系良好,r=0.9996,平均加样回收率为101.48%,RSD为1.07%;β-细辛醚在0.187～4.672 mg·ml^(-1)范围内与峰面积线性关系良好,r=0.9999,平均加样回收率为99.65%.RSD为1.31%。结论:所建立的方法简便、准确,可作为小儿遗尿分散片的质量控制方法。

T Objective： To establish the method for determination of α-asarone and βasarone in children＇ s yiniao dispersible tab- lets. Method： RP-HPLC with Hypersil ODS Cls（250 mm ×4.6 mm,5 μm）column was applied with methanol： water （60： 40） as the mobile phase, detection wavelength at 257 nm, column temperature at 30 ℃ and the flow rate was 1.0 ml-min-1. Result： The linear range of calibration curve was 0. 051-1. 286 mg·ml-1 for α-asarone （r =0. 999 6）. The average recovery was 101.48% and RSD was 1.07%. The linear range of calibration curve was 0. 187- 4. 672 mg· ml-1 for β-asarone （r = 0.999 9）. The average recovery was 99. 65% and RSD was 1.31%. Conclusion： The method is simple and accurate, and can be used as a quality control method for children yiniao dispersible tablets.