低能N^+注入E.coli诱发的HRS/IRR与抗氧化物酶关系的初探

Research on Relation of HRS/IRR and Anti-oxidases in E.coli Implanted by 30 keV N^+

研究了30keV N+注入E.coli诱发的辐射超敏感效应/增强的辐射性(HRS/IRR)及该效应诱发时E.coli细胞内抗氧化物酶(SOD和CAT)的诱导表达,探讨了细胞内抗氧化物酶的诱导与HRS/IRR诱发之间的关联。研究结果显示,HRS诱发剂量范围内(0—5×1014N+/cm2),E.coli细胞内超氧化物岐化酶(SOD)的酶活基本维持在10Us/mg的较低水平,过氧化氢敏(CAT)酶活也都低于8Us/mg;IRR诱发的剂量范围内(5×1014—10×1014N+/cm2),SOD和CAT活性升高并分别达到了58Us/mg和26Uc/mg的最高水平。研究结果初步表明,低能离子注入E.coli HRS/IRR的诱发与细胞内抗氧化物酶的诱导关系密切。

The inducement of hyperradiosensitivity/increased radioresistance（HRS/IRR） and activities of anti-oxidases in Escherichia coli implanted by 30 keV N＋ and relationship between them were investigated. The results showed the change trend of the survival rate and the activity of superoxide dismutase（SOD） and Catalas（CAT） of E. coli K12 was consistent. The activity of SOD and CAT of E. coli were lower, less than 10 and 8 U/mg, when the HRS was induced at 0--5×10^14N^＋/cm^2 doses. And the activity of SOD and CAT of E. coli increased and reached highest rate, were 58 and 26 U/mg respectively, when the IRR was induced at 5 ;4 1014--10×10^14N^＋/cm^2 doses. It could be concluded that the change of SOD and CAT activity was related with the HRS/IRR.